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Published: 2015-08-30

Evaluation of Downstream Regulatory Element Antagonistic Modulator Gene in Human Multinodular Goiter

Amanda Shinzato, Antonio M. Lerario, Chin J. Lin, Debora S. Danilovic, Suemi Marui, Ericka B. Trarbach

(Laboratory of Cellular and Molecular Endocrinology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil)

Med Sci Monit Basic Res 2015; 21:179-182

DOI: 10.12659/MSMBR.895096


BACKGROUND: DREAM (Downstream Regulatory Element Antagonistic Modulator) is a neuronal calcium sensor that was suggested to modulate TSH receptor activity and whose overexpression provokes an enlargement of the thyroid gland in transgenic mice. The aim of this study was to investigate somatic mutations and DREAM gene expression in human multinodular goiter (MNG).
MATERIAL AND METHODS: DNA and RNA samples were obtained from hyperplastic thyroid glands of 60 patients (54 females) with benign MNG. DREAM mutations were evaluated by PCR and direct automatic sequencing, whereas relative quantification of mRNA was performed by real-time PCR. Over- and under-expression were defined as a 2-fold increase and decrease in comparison to normal thyroid tissue, respectively. RQ M (relative quantification mean); SD (standard deviation).
RESULTS: DREAM expression was detected in all nodules evaluated. DREAM mRNA was overexpressed in 31.7% of MNG (RQ M=6.26; SD=5.08), whereas 53.3% and 15% had either normal (RQ M=1.16; SD=0.46) or underexpression (RQ M=0.30; SD=0.10), respectively. Regarding DREAM mutations analysis, only previously described intronic polymorphisms were observed.
CONCLUSIONS: We report DREAM gene expression in the hyperplastic thyroid gland of MNG patients. However, DREAM expression did not vary significantly, and was somewhat underexpressed in most patients, suggesting that DREAM upregulation does not ... read more

Keywords: DNA Mutational Analysis, Gene Expression, Goiter, Nodular, Kv Channel-Interacting Proteins




Published: 2015-08-17

Combination of European System for Cardiac Operative Risk Evaluation (EuroSCORE) and Cardiac Surgery Scor...


Fabian Doerr, Matthias B. Heldwein, Ole Bayer, Anton Sabashnikov, Alexander Weymann, Pascal M. Dohmen, Thorsten Wahlers, Khosro Hekmat

Med Sci Monit Basic Res 2015; 21:172-178

DOI: 10.12659/MSMBR.895004

Published: 2015-08-13

Metabolic and Ventilatory Changes During and After High-Frequency Yoga Breathing


Shirley Telles, Nilkamal Singh, Acharya Balkrishna

Med Sci Monit Basic Res 2015; 21:161-171

ID: 894945