eISSN 2325-4415


Plant-Animal Research Competition

Four Awards ($1,500 each) + publication expenses

We are announcing a competition for external investigator reports dealing with plant-animal communications, which influence animal/human tissues. Eligible studies can vary in their methodology, but state-of-the-art research submissions are encouraged.

Awards Guidelines and Program:
  1. Four Awards granted ($1,500 each) + reimburse of publication expenses
  2. All reports prepared according to the... read more

Published: 2014-12-10

Suitability of the Rat Subdermal Model for Tissue Engineering of Heart Valves

Torsten Christ, Pascal M. Dohmen, Sebastian Holinski, Melanie Schönau, Georg Heinze, Wolfgang Konertz

(Department of Cardiovascular Surgery, Charité-Universitätsmedizin Berlin, Berlin, Germany)

Med Sci Monit Basic Res 2014; 20:194-199

DOI: 10.12659/MSMBR.893088

Background: Tissue engineering (TE) is a promising approach to overcome problems associated with biological heart valve prosthesis. Currently several animal models are used to advance this method. The rat subdermal model is uncomplicated and widely used, but its suitability for TE has not yet been shown.
Material and Methods: Using the rat subdermal model we implanted two decellularized porcine aortic wall specimens (of which one was endothelialized) and one native porcine aortic wall specimen in 30 Lewis rats, respectively. Endothelial cells (EC) were harvested from the rat jugular veins. After explantation Hematoxylin/Eosin-staining, CD-68-positive cell staining, fibroblast-staining and Von-Willebrand factor staining were performed.
Results: All animals survived without complications. Endothelialization was confirmed to be effective by Giemsa staining. Histological evaluation of specimens in Hematoxylin/Eosin staining showed significant decrease (p<0.05) of inflammatory reaction (confirmed by CD-68-positive cell staining) after decellularization. All specimens showed strongest inflammatory reactions at areas of destroyed extracellular matrix. Fibroblasts could be detected in all specimens, with strongest infiltration in decellularized specimens (p<0.05). Surrounding endothelialized specimens had no monolayer of endothelial cells, but a higher density of blood vessels occurred (p<0.05).
Conclusions: The subdermal model provides excellent contact of host tissue with implanted specimens leading to rapid cellular infiltration; therefore, we could ascertain reduced inflammatory response to decellularized tissue. Due... read more

Keywords: Heart Valve Prosthesis, Heart Valves, Models, Animal, Tissue Engineering

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