eISSN 2325-4416


Get your full text copy in PDF

The effects of morphine on Parkinson’s-related genes PINK1 and PARK2

Christopher Snyder, Kirk Mantione

(Neuroscience Research Institute, State University of New York at Old Westbury, Old Westbury, USA)

Med Sci Monit Basic Res 2014; 20:63-69

DOI: 10.12659/MSMBR.890557

Background: Parkinson’s disease (PD) continues to be an important neurological disorder. It is caused by the loss of dopaminergic neurons in the substantia nigra. Dopamine, the neurotransmitter produced from dopaminergic neurons, is a major precursor of endogenous morphine. There are approximately 18 genes associated with PD; their roles have not yet been completely established. PARK2 is a gene that encodes for the protein parkin, and PINK1 is a gene that encodes for PTEN-induced putative kinase 1.
Material and Methods: Our objective was to determine if morphine treatment of HTB-11 cells affects the expression of PINK1 and PARK2. HTB-11 cells were treated with 10–7 M morphine for 2 h and a microarray analysis was conducted. To verify the microarray analysis, 3 Q-PCR trials were run using 10–6 M naloxone, morphine (10–7 M), or a naloxone/morphine mix.
Results: In both the microarray analysis and the Q-PCR analysis, PARK2 was up-regulated and PINK1 was down-regulated.
Conclusions: Morphine can affect the expression of PD-associated genes.

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree