Figure 5 Effect of paclitaxel on palmitate-induced inflammation. Podocytes were treated with 300 μM palmitate with or without paclitaxel for 24 h. (A) Real-time PCR of MCP-1 (top) and TNF-α (bottom) were normalized by β-actin mRNA level in the same sample. Error bar represents the mean±SEM (n=4). (B) TNF-R2 and TLR4 mRNA was normalized by β-actin mRNA level in the same sample. Western blots, densitometry (top, n=3) and mRNA expression are shown (bottom, n=4). Bar represent the mean±SEM. BSA – bovine serum albumin; PA – palmitate; PTX – paclitaxel. ** P<0.01, *** P<0.001 compared with palmitate.