01 September 2007
Can autologous vascular endothelial cell seeding increase the patency rate of small-diameter No-React®-treated bovine internal mammary arteries? An in vivo study in juvenile sheepPascal Maria Dohmen, Francisco da Costa, Sergio Yoschi, Sergio Vega Lopes, Felipe Pohl da Souza, Wolfgang Konertz
Med Sci Monit 2007; 13(9): BR188-193 :: ID: 498331
Background: This study was performed to evaluate the possibility of seeding No-React®-treated bovine small-diameter internal mammary arteries (SIMAs) and to record any improvement in patency rate.
Material/Methods: During the in vitro study, 12 seeded SIMAs were divided into two groups; group I (n=6) was endothelial cell (EC) seeded and group II (n=6) EC seeded after pre-coating with fibrin glue to evaluate the binding capacity. During the in vivo part of the study, eight juvenile sheep received either a seeded or a non-seeded SIMA. In the seeded group (n=3), a piece of jugular vein was harvested to culture autologous ECs. SIMA grafts were coated and seeded in a special bioreactor. In the control group (n=5), non-seeded SIMA grafts were implanted. No anti-coagulation was administered. Explantation was performed at three and six months post-implantation. Grafts were evaluated by gross examination, histology, and immunohistochemistry.
Results: By inserting two million ECs in the in vitro study, the seeding density in group I was 1.29±0.09×10[sup]5[/sup] cells/cm[sup]2[/sup] and 2.27±0.17×10[sup]5[/sup] cells/cm[sup]2[/sup] in group II (p<0.003). In the in vivo study the mean EC density of the implanted seeded grafts was 2.35±0.04×105 cells/cm². At explantation, the seeded grafts showed less infl ammatory reaction and a higher patency rate compared with the non-seeded grafts.Histology showed a monolayer of ECs on the inner surface of seeded SIMAs at follow-up.
Conclusions: Seeding of No-React®-treated SIMA arterial grafts with autologous ECs increases the patency
Keywords: Cattle, Aging - physiology, Cell Culture Techniques - methods, Coculture Techniques - methods, Endothelial Cells - cytology, Mammary Glands, Animal - blood supply, Sheep
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