Logo Medical Science Monitor Basic Research

Call: 1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Contact Us

Logo Medical Science Monitor Basic Research Logo Medical Science Monitor Basic Research Logo Medical Science Monitor Basic Research

01 November 1999

Kupffer cell inhibition and its effect on ischemia/reperfusion injury as well as the biotransforming potential of the liver

Ryszard Wiaderkiewicz, Anna Wiaderkiewicz, Danuta Plewka, Marcin Kamiński, Artur Caban, Grzegorz Oczkowicz, Stanisław Kuśmierski

Med Sci Monit 1999; 5(6): BR1074-1080 :: ID: 503100


Kupffer cells are believed to play an important role during ischemia-reperfusion (IR) injury of the liver, mostly by releasing pro-inflammatory factors and oxygen free radical formation. In our study we investigated the effect of pharmacological Kupffer cell inhibition by gadolinium chloride (GdCl3 Ð a selective Kupffer cell toxicant) on IR injury, as well as the biotransforming potential of the liver intoxicated with the organophosphorous insecticide Ð chlorfenvinphos. The study was performed on rats divided into 6 groups. Group 1 served as the control. Group 2 rats were subjected to 60 min of ischemia followed by 24 hours of reperfusion (I/R). Group 3 rats received chlorfenvinphos (i.p. Ð 2 mg/kg body weight) and were sacrificed after 24 hours. Group 4 rats received chlorfenvinphos after 30 min of ischemia and were sacrificed after 24 hours of reperfusion. Group 5 rats were subjected to I/R and GdCl3 was injected (i.v.) prior to reperfusion. Group 6 comprised rats subjected to I/R, treated with GdCl3 as mentioned above and injected with chlorfenvinphos. Liver sections were evaluated morphologically (H&E staining) and histochemically (SDH, LDH, G6Pase, Mg+2 ATPase and AcP activity). We evaluated the total content of cytochromes P450 and b5, activities of their corresponding NADPH and NADH reductases, as well as the presence of CYP1A1, CYP2E1 and CYP2B1/B2 isoforms in the microsomal fraction of the liver. I/R affects all studied enzyme activities and reduces the cytochrome P450 content and activity of its NADPH reductase. Amongst the studied isoforms, CYP2B1/B2 are mostly affected by IR. Intraperitoneal injection of chlorfenvinfos alone caused slight damage to the liver, although dramatically affected all studied parameters in rats subjected to I/R. The injection of gadolinium chloride alone has no effect on the structure nor biotransforming potential of the liver. However, the injection of GdCl3 prior to reperfusion demonstrated a beneficial effect on the activity of marker enzymes and diminished the morphological liver changes caused by I/R.

Keywords: P450 isoforms, Kupffer cells, gadolinium chloride, Reperfusion, Ischemia


Most Viewed Current Articles

13 Apr 2020 : Original article  

Outcome of 24 Weeks of Combined Schroth and Pilates Exercises on Cobb Angle, Angle of Trunk Rotation, Chest...

DOI :10.12659/MSMBR.920449

Med Sci Monit Basic Res 2020; 26:e920449

11 May 2020 : Original article  

Analysis of Psychological and Sleep Status and Exercise Rehabilitation of Front-Line Clinical Staff in the ...

DOI :10.12659/MSMBR.924085

Med Sci Monit Basic Res 2020; 26:e924085

05 Jan 2021 : Review article  

A Southeast Asian Perspective on the COVID-19 Pandemic: Hemoglobin E (HbE)-Trait Confers Resistance Against...

DOI :10.12659/MSMBR.929207

Med Sci Monit Basic Res 2021; 27:e929207

10 Aug 2020 : Clinical Research  

Effects of Cognitive Task Training on Dynamic Balance and Gait of Patients with Stroke: A Preliminary Rando...

DOI :10.12659/MSMBR.925264

Med Sci Monit Basic Res 2020; 26:e925264

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416