25 July 2013
Med Sci Monit Basic Res 2013; 19:201-207
The purpose of this study was to investigate the role of activated Rho GTPase cell division control protein 42 homolog (Cdc42) in colorectal cancer cell adhesion, migration, and invasion.
Material and Methods
The constitutively active form of Cdc42 (GFP-Cdc42L61) or control vector was overexpressed in the colorectal cancer cell line SW480. The localization of active Cdc42 was monitored by immunofluorescence staining, and the effects of active Cdc42 on cell migration and invasion were examined using an attachment assay, a wound healing assay, and a Matrigel migration assay in vitro.
Immunofluorescence staining revealed that constitutively active Cdc42 predominately localized to the plasma membrane. Compared to SW480 cells transfected with the control vector, overexpression of constitutively active Cdc42 in SW480 cells promoted filopodia formation and cell stretch and dramatically enhanced cell adhesion to the coated plates. The wound healing assay revealed a significant increase of migration capability in SW480 cells expressing active Cdc42 compared to the control cells. Additionally, the Matrigel invasion assay demonstrated that active Cdc42 significantly promoted SW480 cell migration through the chamber.
Our results suggest that active Rho GTPase Cdc42 can greatly enhance colorectal cancer cell SW480 to spread, migrate, and invade, which may contribute to colorectal cancer metastasis.
Keywords: Focal Adhesions - metabolism, Colorectal Neoplasms - pathology, Cell Adhesion, cdc42 GTP-Binding Protein - metabolism
Most Viewed Current Articles
15 Jun 2022 : Clinical ResearchEvaluation of Apical Leakage After Root Canal Obturation with Glass Ionomer, Resin, and Zinc Oxide Eugenol ...
Med Sci Monit Basic Res 2022; 28:e936675
07 Jul 2022 : Laboratory ResearchCytotoxicity, Apoptosis, Migration Inhibition, and Autophagy-Induced by Crude Ricin from Ricinus communis S...
Med Sci Monit Basic Res 2022; 28:e936683
01 Jun 2022 : Laboratory ResearchComparison of Sealing Abilities Among Zinc Oxide Eugenol Root-Canal Filling Cement, Antibacterial Biocerami...
Med Sci Monit Basic Res 2022; 28:e936319
17 Jan 2022 : Clinical ResearchAnodal Transcranial Direct Current Stimulation (tDCS) Over the Primary Motor Cortex (M1) Enhances Motor Res...
Med Sci Monit Basic Res 2022; 28:e934180