Renalase mRNA levels in the brain, heart, and kidneys of spontaneously hypertensive rats with moderate and high hypertension
Valerii Fedchenko, Alexander Globa, Olga Buneeva, Alexei Medvedev
Moscow , Russian Federation
Med Sci Monit Basic Res 2013; 19:267-270
Renalase is a recently discovered secretory protein involved in regulation of arterial blood pressure in humans and animals. Results of animal experiments from independent laboratories indicate that administration of human recombinant renalase decreases blood pressure and some genetically predisposed hypertensive rats have lowered renalase levels.
Material and Methods: The levels of renalase mRNA expression in brain hemispheres, heart, and kidneys of spontaneously hypertensive rats (SHR) with moderate (140–180 mm Hg) or high (>180 mm Hg) hypertension and of control Wistar-Kyoto (WKY) rats were analyzed using real-time PCR.
Results: Spontaneously hypertensive rats with high hypertension (>180 mm Hg) had a lower renalase mRNA level in brain hemispheres, and higher heart and kidney renalase mRNA levels compared with control WKY rats. In SHR with a moderate increase in arterial blood pressure (140–180 mm Hg), the tissue renalase mRNA changed in the same direction but did not reach the level of statistical significance as compared with control rats.
Conclusions: The results indicate that the development of hypertension in SHR is accompanied by altered expression of the renalase gene in the examined organs as compared with control WKY rats. The brain and peripheral tissues renalase mRNA levels demonstrate opposite trends, which are obviously crucial for impaired regulation of blood pressure in SHR.
Keywords: Gene Expression Regulation, Enzymologic, Brain - enzymology, Animals, Hypertension - genetics, Kidney - enzymology, Monoamine Oxidase - metabolism, Myocardium - enzymology, RNA, Messenger - metabolism, Rats, Rats, Inbred SHR, Real-Time Polymerase Chain Reaction