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Effect of pheniramine maleate on reperfusion injury in brain tissue

Ismail Yürekli, Orhan Gökalp, Müge Kiray, Gamze Gökalp, Kazım Ergüneş, Ebru Salman, Banu Şarer Yürekli, Ismail Safa Satoğlu, Yüksel Beşir, Habib Çakır, Ali Gürbüz

Izmir, Turkey

Med Sci Monit Basic Res 2013; 19:285-290

DOI: 10.12659/MSMBR.889570

Available online:

Published: 2013-12-06


Background: The aim of this study was to investigate the protective effects of methylprednisolone (Pn), which is a potent anti-inflammatory agent, and pheniramine maleate (Ph), which is an antihistaminic with some anti-inflammatory effects, on reperfusion injury in brain developing after ischemia of the left lower extremity of rats.
Material and Methods: Twenty-eight randomly selected male Sprague-Dawley rats were divided into 4 groups: Group 1 was the control group, Group 2 was the sham group (I/R), Rats in Group 3 were subjected to I/R and given Ph, and rats in Group 4 were subjected to I/R and given Pn. A tourniquet was applied at the level of left groin region of subjects in the I/R group after induction of anesthesia. One h of ischemia was performed with no drug administration. In the Ph group, half of a total dose of 10 mg/kg Ph was administered intraperitoneally before ischemia and the remaining half before reperfusion. In the Pn group, subjects received a single dose of 50 mg/kg Pn intraperitoneally at the 30th min of ischemia. Brains of all subjects were removed after 24 h for examination.
Results: Malondialdehyde (MDA) levels of the prefrontal cortex were significantly lower in the Ph group than in the I/R group (p<0.05). Superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities were found to be significantly higher in the Ph group than in the I/R group (p<0.05). Histological examination demonstrated that Ph had protective effects against I/R injury developing in the brain tissue.
Conclusions: Ph has a protective effect against ischemia/reperfusion injury created experimentally in rat brains.

Keywords: Brain Injuries - metabolism, Animals, Histamine H1 Antagonists - therapeutic use, In Situ Nick-End Labeling, Malondialdehyde - metabolism, Pheniramine - therapeutic use, Rats, Rats, Sprague-Dawley, Reperfusion Injury - drug therapy