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07 May 2014 : Original article  

The effects of morphine on Parkinson’s-related genes PINK1 and PARK2

Christopher SnyderABCDEF, Kirk MantioneACDEF

DOI: 10.12659/MSMBR.890557

Med Sci Monit Basic Res 2014; 20:63-69

Abstract

BACKGROUND: Parkinson’s disease (PD) continues to be an important neurological disorder. It is caused by the loss of dopaminergic neurons in the substantia nigra. Dopamine, the neurotransmitter produced from dopaminergic neurons, is a major precursor of endogenous morphine. There are approximately 18 genes associated with PD; their roles have not yet been completely established. PARK2 is a gene that encodes for the protein parkin, and PINK1 is a gene that encodes for PTEN-induced putative kinase 1.

MATERIAL AND METHODS: Our objective was to determine if morphine treatment of HTB-11 cells affects the expression of PINK1 and PARK2. HTB-11 cells were treated with 10–7 M morphine for 2 h and a microarray analysis was conducted. To verify the microarray analysis, 3 Q-PCR trials were run using 10–6 M naloxone, morphine (10–7 M), or a naloxone/morphine mix.

RESULTS: In both the microarray analysis and the Q-PCR analysis, PARK2 was up-regulated and PINK1 was down-regulated.

CONCLUSIONS: Morphine can affect the expression of PD-associated genes.

Keywords: Gene Expression Regulation - drug effects, Morphine - pharmacology, Oligonucleotide Array Sequence Analysis, Parkinson Disease - genetics, Protein Kinases - metabolism, Real-Time Polymerase Chain Reaction, Ubiquitin-Protein Ligases - metabolism

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Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416