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Brain, Liver, and Serum Salusin-alpha and -beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome

Cihan Citil, Vahit Konar, Suleyman Aydin, Musa Yilmaz, Serdal Albayrak, Ibrahim Hanifi Ozercan, Yusuf Ozkan

Atatürk Vocational School of Health Science, Kafkas University, Kars, Turkey

Med Sci Monit 2014; 20:1326-1333

DOI: 10.12659/MSM.890708

Available online:

Published: 2014-07-29

Background: This metabolic syndrome (MetS) study was designed to investigate changes in expression of the neuropeptides salusin-α (Sal-α) and salusin-β (Sal-β) in brain and liver tissue in response to obesity and related changes induced by high-fructose diet and explored how these changes were reflected in the circulating levels of Sal-α and Sal-b, as well as revealing how the lipid profile and concentrations of glucose and uric acid were altered.
Material and Methods: The study included 14 Sprague-Dawley rats. The control group was fed ad libitum on standard rat pellets, while the intervention group was given water with 10% fructose in addition to the standard rat pellet for 3 months. Sal-α and Sal-β concentrations in the serum and tissue supernatants were measured by ELISA, and immunohistochemical staining was used to demonstrate expression of the hormones in brain and liver.
Results: Sal-α and Sal-β levels in both the serum and the brain and liver tissue supernatants were lower in the MetS group than the control group. Sal-α and Sal-β were shown by immunohistochemistry to be produced in the brain epithelium, the supraoptic nucleus of the hypothalamus, and the liver hepatocytes.
Conclusions: The decrease in Sal-α and Sal-β might be involved in the etiopathology of the metabolic syndrome induced by fructose.

Keywords: Brain - metabolism, Animals, Enzyme-Linked Immunosorbent Assay, Fructose, Intercellular Signaling Peptides and Proteins - metabolism, Liver - metabolism, Metabolic Syndrome X - metabolism, Obesity - metabolism, Rats, Rats, Sprague-Dawley, Statistics, Nonparametric