Aberrant Methylation of Protocadherin 17 and its Clinical Significance in Patients with Prostate Cancer after Radical Prostatectomy
Ying-Li Lin, Pei-Gen Xie, Li Wang, Jian-Guo Ma
Department of Urology, Xuzhou Cancer Hospital (Affiliated Xuzhou Hospital, Jiangsu University), Xuzhou, China (mainland)
Med Sci Monit 2014; 20:1376-1382
Aberrant methylation of protocadherin 17 (PCDH17) has been reported in several human cancers. However, the methylation status of PCDH17 in prostate cancer and its clinical significance remains unclear. The aim of this study was to investigate the methylation status of PCDH17 and its clinical significance in patients with prostate cancer after radical prostatectomy.
Material and Methods: The methylation status of PCDH17 in 152 prostate cancer tissues and 51 non-tumoral prostate tissues was examined by methylation-specific PCR (MSP). Then the association between PCDH17 methylation and clinicopathologic parameters was analyzed. Kaplan-Meier survival analysis, log-rank test and multivariate Cox proportional hazard model analysis were used to analyze the correlation between PCDH17 methylation and prognosis of patients with prostate cancer.
Results: Our data demonstrated that PCDH17 methylation occurred frequently in prostate cancer. PCDH17 methylation was significantly associated with higher pathological Gleason score (P=0.0315), advanced pathological stage (P=0.0260), higher level of preoperative PSA (P=0.0354), positive angiolymphatic invasion (P=0.0461), positive lymph node metastasis (P=0.0362), and biochemical recurrence (BCR) (P=0.0018). In addition, PCDH17 methylation was an independent predictor of poor biochemical recurrence-free (BCR-free) survival and overall survival for patients with prostate cancer.
Conclusions: PCDH17 methylation is a frequent tumor-specific event in prostate cancer, and is significantly correlated with shorter BCR-free survival and overall survival of patients with prostate cancer after radical prostatectomy. PCDH17 methylation in tumor samples after radical prostatectomy may be used as an independent prognostic biomarker.
Keywords: Aged, 80 and over, Cadherins - metabolism, China, DNA Methylation - genetics, DNA Primers - genetics, Disease-Free Survival, Kaplan-Meier Estimate, Polymerase Chain Reaction, Proportional Hazards Models, Prostate-Specific Antigen - blood, Prostatectomy, Prostatic Neoplasms - surgery, Tumor Markers, Biological - genetics