Marie Godfrin-Valnet, Kashif A. Khan, Xavier Guillot, Clément Prati, Lucile Baud, Wasim Abbas, Eric Toussirot, Daniel Wendling, Georges Herbein
Department of Rheumatology, Centre Hospitalier Régional Universitaire (CHRU) Besançon, Besançon, France
Med Sci Monit Basic Res 2014; 20:142-145
Sirtuin 1 (SIRT1) is a class III histone deacetylase that may play a critical role in several biological functions, including lifespan, stress, and inflammation. Our main objective was to evaluate SIRT1 activity in peripheral blood mononuclear cells (PBMCs) in patients with osteoporosis and to analyze the relationship between the SIRT 1 activity and markers of inflammation and bone remodelling.
Material and Methods: We performed a prospective monocentric study of patients with osteoporosis and measured the nuclear and cytoplasmic activities of SIRT1 in PBMCs. Levels of proinflammatory cytokines were assessed in culture supernatants of PBMCs isolated from the osteoporosis patients. The level of serum C-terminal cross-linking telopeptide of type I collagen (CTX), a marker of bone resorption, was measured in the serum of osteoporosis patients.
Results: Sixteen women with osteoporosis were included. A statistically significant correlation between the cytoplasmic and nuclear SIRT 1 activities was found in PBMCs of patients with osteoporosis. Although non-significant, we observed a negative trend between nuclear SIRT 1 activity and the rate of serum CTX and a positive trend between IL-6 and CTX levels in patients with osteoporosis.
Conclusions: This study shows that the cytoplasmic and nuclear SIRT 1 activities are measurable in circulating PBMCs of patients with osteoporosis and that these 2 activities are correlated. The potential role of inflammation in bone resorption in patients with osteoporosis was also studied.
Keywords: Case-Control Studies, Cell Nucleus - metabolism, Collagen Type I - blood, Interleukin-6 - blood, Leukocytes, Mononuclear - enzymology, Osteoporosis - enzymology, Peptides - blood, Sirtuin 1 - blood