İbrahim Kaplan, Enver Sancaktar, Aydın Ece, Velat Şen, Nilgün Tekkeşin, Mustafa Kemal Basarali, Selvi Kelekci, Osman Evliyaoglu
Department of Medical Biochemistry, Dicle University, Diyarbakir, Turkey
Med Sci Monit 2014; 20:1745-1750
Hypertension is a major global public health problem that affects both pediatric and adult populations. ACE I/D, AGT M235T, and ADD Gly460Trp polymorphisms are thought to be associated with primary hypertension. In the present study, we examined the frequency of these polymorphisms in a pediatric population with secondary hypertension.
Material and Methods: Included in the study were 58 hypertensive and 58 normotensive pediatric patients. ACE I/D and AGT M235T polymorphisms are determined by conventional PCR; ADD Gly460Trp polymorphism was investigated using PCR amplification of genomic DNA.
Results: There were significant differences between the control group and pediatric hypertensive group in terms of ACE I/D (P<0.05) and AGT M235T (P<0.05) polymorphisms, but there were no differences in ADD Gly460Trp (P>0.05) polymorphism.
Conclusions: We suggest that RAS gene polymorphisms (ACE-I/D, AGT M235T) are significantly associated with susceptibility to diseases that lead to secondary hypertension.
Keywords: Angiotensinogen - genetics, Alleles, Calmodulin-Binding Proteins - genetics, Case-Control Studies, Child, Gene Frequency, Genetic Predisposition to Disease, Hypertension - genetics, Peptidyl-Dipeptidase A - genetics, Polymorphism, Single Nucleotide - genetics, Renal Insufficiency, Chronic - genetics