Proliferation and Differentiation of Rat Osteoporosis Mesenchymal Stem Cells (MSCs) after Telomerase Reverse Transcriptase (TERT) Transfection
Chao Li, Guojun Wei, Qun Gu, Qiang Wang, Shuqin Tao, Liang Xu
Department of Orthopaedics, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland)
Med Sci Monit 2015; 21:845-854
Available online: 2015-03-22
The aim of this study was to determine whether MSC are excellent materials for MSCs transplantation in the treatment of osteoporosis.
MATERIAL AND METHODS: We studied normal, osteoporosis, and TERT-transfected MSC from normal and osteoporosis rats to compare the proliferation and osteogenic differentiation using RT-PCR and Western blot by constructing an ovariectomized rat model of osteoporosis (OVX). The primary MSC from model rats were extracted and cultured to evaluate the proliferation and differentiation characteristics.
RESULTS: MSCs of osteoporosis rats obviously decreased in proliferation ability and osteogenic differentiation compared to that of normal rats. In contrast, in TERT-transfected MSC, the proliferation and differentiation ability, and especially the ability of osteogenic differentiation, were significantly higher than in osteoporosis MSC.
CONCLUSIONS: TERT-transfected MSCs can help osteoporosis patients in whom MSC proliferation and osteogenic differentiation ability are weak, with an increase in both bone mass and bone density, becoming an effective material for autologous transplantation of MSCs in further treatment of osteoporosis. However, studies are still needed to prove the in vivo effect, biological safety, and molecular mechanism of TERT-osteoporosis treatment. Additionally, because the results are from an animal model, more research is needed in generalizing rat model findings to human osteoporosis patients.
Keywords: Blotting, Western, Antigens, CD - metabolism, Animals, Cell Differentiation, Disease Models, Animal, Femur - pathology, Gene Expression Regulation, Genetic Vectors - metabolism, Lentivirus - metabolism, Mesenchymal Stromal Cells - pathology, Osteoporosis - pathology, Ovariectomy, Plasmids - metabolism, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction, Telomerase - metabolism, Transfection