Expression Level of Genes Coding for Cell Adhesion Molecules of Cadherin Group in Colorectal Cancer Patients
Zbigniew Lorenc, Mieszko Norbert Opiłka, Celina Kruszniewska-Rajs, Antoni Rajs, Dariusz Waniczek, Małgorzata Starzewska, Justyna Lorenc, Urszula Mazurek
Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland
Med Sci Monit 2015; 21:2031-2040
Colorectal Cancer (CRC) is one of the most frequently diagnosed neoplasms and also one of the main death causes. Cell adhesion molecules are taking part in specific junctions, contributing to tissue integrality. Lower expression of the cadherins may be correlated with poorer differentiation of the CRC, and its more aggressive phenotype. The aim of the study is to designate the cadherin genes potentially useful for the diagnostics, prognostics, and the treatment of CRC.
MATERIAL AND METHODS: Specimens were collected from 28 persons (14 female and 14 male), who were operated for CRC. The molecular analysis was performed using oligonucleotide microarrays, mRNA used was collected from adenocarcinoma, and macroscopically healthy tissue. The results were validated using qRT-PCR technique.
RESULTS: Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression. The control group was divided into 2 groups, the one was appropriate control (C1), the second (C2) had the genetic properties of the CRC, without pathological changes histologically and macroscopically. The other 2 groups were: LSC (Low stage cancer) and HSC (High stage cancer). Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue.
CONCLUSIONS: The levels of genes expression are different for several groups of cadherins, and are related with the stage of CRC, therefore could be potentially the useful marker of the stage of the disease, also applicable in treatment and diagnostics of CRC.
Keywords: Colorectal Neoplasms - surgery, Cell Adhesion Molecules - genetics, Cadherins - genetics, Gene Expression Regulation, Neoplastic