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16 June 2015 : Original article  

Use of X-Chromosome Inactivation Pattern to Analyze the Clonality of 14 Female Cases of Kaposi Sarcoma

Ding YuanE, Wu XiuJuanC, Zhang YanD, Liang JunQinD, Xiang FangB, Yu ShirongB, Kang XiaojingA, Feng YanyanD, Wu WeidongA, Luo DongF, Lu QingliF, Zhang DeZhiB, Pu XiongMingAG

DOI: 10.12659/MSMBR.894089

Med Sci Monit Basic Res 2015; 21:116-122

Abstract

BACKGROUND: Kaposi sarcoma (KS) has features of both neoplastic growth and hyperplastic proliferation. It is the most common tumor seen in patients with HIV infection. Whether KS is a real tumor or a benign hyperplastic disease is not known.

MATERIAL AND METHODS: Tissues from KS and cutaneous hemangioma lesion DNA were extracted, and then digested with methylation-sensitive restriction endonuclease HpaII. Human androgen receptor gene (HUMARA) was amplified with PCR method and the product was separated on 10% denaturing polyacrylamide gels and stained with ethylene dibromide (EB) to show the polymorphism of HUMARA. Phosphoglycerate kinase (PGK) was amplified and the product was digested by BStXI, agarose gel and EB stained to show the polymorphism of PGK. Finally, we analyzed the clonality of KS.

RESULTS: In the 14 patients with KS, heterozygosity of the HUMARA gene was observed in 12 (85.7%) cases. Loss of heterozygosity of HUMARA gene on X-chromosome (without HpaII digestion there were 2 bands, after HpaII digestion there were just 1 of the bands), representing monoclonal origin, was present in 11 cases of Kaposi sarcoma. Heterozygosity of the PGK gene was observed in 5 (35.7%) cases, which all represent monoclonal origin. There was no significant difference according to country, stage, or HIV and HHV-8 (P>0.05).

CONCLUSIONS: The current findings suggest that Kaposi sarcoma is a clonal neoplasm, not a reactive proliferation.

Keywords: DNA Primers - genetics, HIV Infections - complications, Loss of Heterozygosity, Nucleic Acid Amplification Techniques, Phosphoglycerate Kinase - metabolism, Polymorphism, Genetic, Receptors, Androgen - metabolism, Sarcoma, Kaposi - genetics, X Chromosome Inactivation - genetics

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Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416