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MiR-29 Induces K562 Cell Apoptosis by Down-Regulating FoxM1

Xiaofang Wang, Hua Zhong, Lei Wang, Yuqian Dong, Ankui Jia, Qingjiang Mo, Chenguang Zhang

Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China (mainland)

Med Sci Monit 2015; 21:3115-3120

DOI: 10.12659/MSM.894554

Available online:

Published: 2015-10-15

BACKGROUND: Leukemia seriously threatens human life and health. MicroRNAs can regulate cell growth, proliferation, and death. This article investigated the role of miR-29 on regulating leukemia cell growth, proliferation, and apoptosis.
MATERIAL AND METHODS: miR-29 and scramble miRNA were transfected to K562 cells. MTT assay, colony formation assay, caspase-3 activity detection, and flow cytometry were applied to test miR-29 effect on cell growth, proliferation, and apoptosis. Western blot was used to detect Forkhead box protein M1 (FoxM1) protein expression. After we transfected miR-29, K562 cells were transfected with FoxM1 siRNA to test cell apoptosis.
RESULTS: K562 cell growth and proliferation were inhibited after transfection with miR-29. Apoptosis phenome and caspase-3 activation were observed. FoxM1 level decreased. SiRNA FoxM1 enhanced miR-29-induced K562 cell apoptosis. FoxM1 overexpression suppressed miR-26-induced K562 cell apoptosis.
CONCLUSIONS: MiR-29 restrained K562 cell growth and proliferation. MiR-29 induced K562 cell apoptosis through down-regulating FoxM1.

Keywords: Caspase 3 - metabolism, Apoptosis, Cell Proliferation, Down-Regulation, Enzyme Activation, Flow Cytometry, Forkhead Transcription Factors - metabolism, Gene Expression Regulation, Leukemic, K562 Cells, MicroRNAs - metabolism, RNA, Messenger - metabolism, Transfection