Junnan Wang, Rihao Xu, Junduo Wu, Zhibo Li
Department of Cardiovascular Surgery, The Second Hospital of Jilin University, Changchun, Jilin, China (mainland)
Med Sci Monit 2015; 21:3498-3504
Oxidative stress, inducing cardiomyocyte apoptosis or myocardial ischemia, is the major denominator of many cardiac diseases. In this study, we intended to explore the regulatory function of microRNA-137 (miR-137) in oxidative stress-induced cardiomyocyte apoptosis.
MATERIAL AND METHODS: Cardiomyocytes were extracted from newborn C57BL/6 mice and cultured in vitro. Apoptosis was induced by H2O2, and evaluated by TUNEL assay. The effect of cardiomyocyte apoptosis on gene expression of miR-137 was evaluated by qRT-PCR. Lentivirus was used to stably down-regulate miR-137, and the subsequent effects of miR-137 down-regulation on cardiomyocyte apoptosis, its targeted gene CDC42, and caspase pathway were evaluated by TUNEL assay, dual-luciferase reporter assay, and Western blot assay, respectively. Finally, CDC42 was down-regulated by siRNA and its effect on miR-137-mediated cardiomyocyte apoptosis protection was examined.
RESULTS: H2O2 induced significant apoptosis and up-regulated miR-137 in cardiomyocytes, whereas lentivirus-mediated miR-137 down-regulation protected against apoptosis. CDC42 was the direct target gene of miR-137 and proteins of CDC42, caspase-3, and caspase-9 were all regulated by miR-137 down-regulation in cardiomyocyte apoptosis. SiRNA-mediated CDC42 down-regulation reversed the protection of miR-137 down-regulation against cardiomyocyte apoptosis.
CONCLUSIONS: Our work demonstrated miR-137 and CDC42 are critical regulators in cardiomyocyte apoptosis. It may help to identify the molecular targets to prevent myocardial injury in human patients.
Keywords: Apoptosis - genetics, Animals, Cells, Cultured, Hydrogen Peroxide - pharmacology, Mice, Mice, Inbred C57BL, MicroRNAs - metabolism, Myocardial Ischemia - pathology, Myocytes, Cardiac - metabolism, Oxidative Stress - drug effects, Up-Regulation - drug effects, cdc42 GTP-Binding Protein - metabolism