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Induction Function of miR-126 in Survival and Proliferation in Neural Stem Cells

Qijun Zhang, Sheng Zeng, Chengyuan Quan, Xiaopo Lin

Department of Emergency, Pingyang People’s Hospital, Wenzhou, Zhejiang, China (mainland)

Med Sci Monit 2015; 21:3023-3027

DOI: 10.12659/MSM.894672

Available online:

Published: 2015-10-07

BACKGROUND: The aim of this study was to investigate the potential function of miR-126 in neural stem cells (NSCs).
MATERIAL AND METHODS: Expression level of miR-126 was detected by quantitative real-time PCR (qRT-PCR). MiR-126 overexpression was established by transfecting miR-126 mimics into human NSC lines (HB1.F3 and HB1.A4 cells). Its effects on cell proliferation were studied using cell-counting kit-8 (CCK8) assay, colony formation assays. Flow cytometry was performed to evaluate the effect of miR-126 on cell survival.
RESULTS: CCK8 assay and colony formation assay showed that overexpression of miR-126 promoted cell proliferation and increased colony numbers in HB1.F3 and HB1.A4 cells. The flow cytometry confirmed the results that miR-126 inhibited cell apoptosis.
CONCLUSIONS: MiR-126 promoted the proliferation and survival of NSCs.

Keywords: Brain - physiology, Apoptosis, Cell Line, Cell Proliferation, Cell Survival, Flow Cytometry, Inflammation, Lentivirus - genetics, MicroRNAs - metabolism, Neural Stem Cells - cytology, Real-Time Polymerase Chain Reaction, Signal Transduction