Weiguo Dong, Wanqing Quo, Feng Wang, Changzheng Li, Yongcai Xie, Xuehua Zheng, Hong Shi
Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland)
Med Sci Monit 2015; 21:3356-3362
Abnormalities of brain energy metabolism are involved in Alzheimer disease (AD). Sirtuin 1 (SIRT1) is a class III histone deacetylase and activates peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α), which enhances mitochondrial biogenesis and energy homeostasis. Electroacupuncture (EA) has been reported to improve brain energy metabolism in AD. However, the effect of EA on SIRT1 and PGC-1α in AD remains unclear.
MATERIAL AND METHODS: ATP levels were measured using assay kits in the hippocampus and frontal cortex of senescence-accelerated mouse prone 8 (SAMP8) mice. Western blotting analysis and quantitative real-time RT-PCR were performed to measure the expression of SIRT1 and PGC-1a in the hippocampus of SAMP8 mice. PGC-1α acetylation was analyzed using immunoprecipitation.
RESULTS: Compared with senescence-accelerated resistant mice 1 (SAMR1) mice, SAMP8 mice had a decline in ATP levels and the expression of SIRT1 and PGC-1α. EA treatment improved ATP levels, upregulated the expression of SIRT1 and PGC-1α, and decreased PGC-1α acetylation.
CONCLUSIONS: These data suggest that EA improved brain energy metabolism, potentially associated with the upregulation of SIRT1-dependent PGC-1α expression.
Keywords: Adenosine Triphosphate - chemistry, Acetylation, Alzheimer Disease - metabolism, Animals, Disease Models, Animal, Electroacupuncture, Frontal Lobe - metabolism, Gene Expression Regulation, Hippocampus - metabolism, Homeostasis, Immunoprecipitation, Mice, Sirtuin 1 - metabolism, Transcription Factors - metabolism, Up-Regulation