Xiaogang Liu, Lihong Ma, Qinhua Rao, Yuhui Mao, Yuhong Xin, Huiqin Xu, Changju Li, Xiaoyan Wang
Department of Gynaecology and Obstetrics, People’s Hospital of Yuxi, Yuxi, Yunnan, China (mainland)
Med Sci Monit 2015; 21:3152-3158
Ovarian cancer is the most lethal gynecological malignant cancer in the female genital system. The dysfunction of miRNA contributes to ovarian cancer development.
MATERIAL AND METHODS: The miR-1271 level in ovarian cancer tissues and cells was assayed by qRT-PCR. The miR-1271 expression in cells was overexpressed by miRNA-mimic transfection and reduced by miRNA-antisense-oligonucleotide (ASO) transfection. Cell proliferation was analyzed by an MTT assay. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. The protein level was assayed by Western blotting.
RESULTS: The ovarian cancer tissue and cell lines showed low levels of miR-1271. Low levels of miR-1271 in ovarian cancer tissues were correlated with a low rate of patient survival, and the overexpression of miR-1271 inhibited the proliferation of ovarian cancer cells. The 3’ UTR of cyclin G1 (CCNG1) was targeted by miR-1271.
CONCLUSIONS: Low levels of miR-1271 in ovarian cancer tissues promoted cancer cell growth. MiR-1271 may be a new predictor of prognosis in ovarian cancer. MiR-1271 exerted its role by targeting CCNG1.
Keywords: Adult, 3' Untranslated Regions, Algorithms, Cadherins - metabolism, Cell Proliferation, Cyclin G1 - metabolism, Gene Expression Regulation, Neoplastic, MicroRNAs - metabolism, Ovarian Neoplasms - metabolism, Prognosis