09 December 2015 : Clinical Research
Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
Ji-shun ZhengABCDEF, Meng-meng ChenBF, Hai-fei YangC, Xiang-tian ZhouBC, Yan-yan LiuB, Jia-bin LiAFGDOI: 10.12659/MSM.896243
Med Sci Monit 2015; 21:3840-3846
Abstract
BACKGROUND: Serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) liver function in patients with chronic hepatitis B (CHB) are significantly associated. A comparison of clinical significance of fecal HBV DNA and serum HBV DNA has not yet been reported.
MATERIAL AND METHODS: Stool and serum samples were collected from 66 patients with CHB. Fecal HBV DNA, serum HBV DNA, and intestinal microbiota DNA were detected by real-time quantitative fluorescence polymerase chain reaction (PCR). Liver function and HBeAg were analyzed.
RESULTS: The stool and serum HBV DNA were positively correlated (r=0.57, P=0.001). Fecal HBV DNA was higher in the HBeAg-positive group than in the HBeAg-negative group (P=0.02). Fecal HBV DNA was negatively correlated with alkaline phosphatase (ALP) (r=–0.41, P=0.001) and TBIL (r=–0.29, P=0.02), and was positively correlated with Enterococcus (r=0.38, P=0.002). Serum HBV DNA was negatively correlated with alanine aminotransferase (ALT) (r=–0.30,P=0.02), aminotransferase (AST) (r=–0.26, P=0.049), and Lactobacillus (r=–0.31, P=0.01).
CONCLUSIONS: These observations suggest that fecal HBV DNA and serum HBV DNA in patients with CHB have different effects. Fecal HBV DNA might be associated with changes in Enterococcus concentrations, but serum HBV DNA is not.
Keywords: DNA, Viral - blood, Feces - virology, Hepatitis B virus - genetics, Hepatitis B, Chronic - virology, Intestines - microbiology, Liver Function Tests, Polymerase Chain Reaction
Most Viewed Current Articles
13 Apr 2020 : Original article
Outcome of 24 Weeks of Combined Schroth and Pilates Exercises on Cobb Angle, Angle of Trunk Rotation, Chest...DOI :10.12659/MSMBR.920449
Med Sci Monit Basic Res 2020; 26:e920449
11 May 2020 : Original article
Analysis of Psychological and Sleep Status and Exercise Rehabilitation of Front-Line Clinical Staff in the ...DOI :10.12659/MSMBR.924085
Med Sci Monit Basic Res 2020; 26:e924085
05 Jan 2021 : Review article
A Southeast Asian Perspective on the COVID-19 Pandemic: Hemoglobin E (HbE)-Trait Confers Resistance Against...DOI :10.12659/MSMBR.929207
Med Sci Monit Basic Res 2021; 27:e929207
10 Aug 2020 : Clinical Research
Effects of Cognitive Task Training on Dynamic Balance and Gait of Patients with Stroke: A Preliminary Rando...DOI :10.12659/MSMBR.925264
Med Sci Monit Basic Res 2020; 26:e925264