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Tumor Suppressor Role of miR-363-3p in Gastric Cancer

Bo Song, Jin Yan, Chao Liu, Haiyang Zhou, Yangchun Zheng

Division of Intestinal Surgery, Department of Abdominal Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan, China (mainland)

Med Sci Monit 2015; 21:4074-4080

DOI: 10.12659/MSM.896556

Available online:

Published: 2015-12-28


BACKGROUND: Gastric cancer (GC) is the most common cancer in the world. Despite the advancement of the treatment of GC, the 5-year overall survival rate is still very low. MicroRNAs (miRNAs) play important roles in the pathogenesis of GC. A recent study suggested that miR-363-3p plays a role in the development of GC. However, the function of miR-363-3p in GC is not fully understood.
MATERIAL AND METHODS: The network of NOTCH1 and the involved molecules was constructed by use of Cytoscape software. MiR-363-3p levels in GC tissues and cells were tested by qRT-PCR. Cells were miR-363-3p mimics or anti-miR-363-3p transfected by Lipofectamine. Bioinformatics algorithms from TargetScanHuman were used to predict the target genes of miR-363-3p. The NOTCH1 protein level was tested by Western blot. The interaction between miR-363-3p and NOTCH1 was confirmed by dual luciferase assays.
RESULTS: MiR-363-3p showed low levels in GC tissues and cells. Enforced expression of miR-363-3p inhibited cell growth and migration of GC cells and vice versa. NOTCH1 is the targeted gene of miR-363-3p.
CONCLUSIONS: MiR-363-3p plays a tumor suppressor role in GC.

Keywords: Cell Cycle - genetics, Adult, Cell Growth Processes - genetics, Cell Line, Tumor, Cell Movement - genetics, Gene Expression Regulation, Neoplastic, MicroRNAs - metabolism, Neoplasm Invasiveness, Receptor, Notch1 - metabolism, Stomach Neoplasms - pathology



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