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Genetic Polymorphisms of SP-A, SP-B, and SP-D and Risk of Respiratory Distress Syndrome in Preterm Neonates

Hong-Yu Chang, Fang Li, Feng-Sheng Li, Cheng-Zhong Zheng, Yan-Zhe Lei, Jing Wang

Department of Pediatrics, The General Hospital of the PLA Rocket Force, Beijing, China (mainland)

Med Sci Monit 2016; 22:5091-5100

DOI: 10.12659/MSM.898553

Available online:

Published: 2016-12-24


BACKGROUND: We examined selected polymorphisms in 3 pulmonary surfactant-associated proteins (SP) for their influence on serum SP levels and risk of respiratory distress syndrome (RDS) in preterm neonates.
MATERIAL AND METHODS: Premature infants from a Han population were enrolled, including 100 premature infants with RDS (case group) and 120 premature infants without RDS (control group). SNP genotyping for SP-A (+186A/G and +655C/T), SP-B (–18A/C and 1580C/T), and SP-D (Met11ThrT/C and Ala160ThrG/A) used polymerase chain reaction-restriction fragment length polymorphism. Haplotypes were calculated with Shesis software and serum SP-A/B/D levels were quantified by ELISA.
RESULTS: Case and control groups exhibited significant differences in genotype and allele frequencies of SP-A (+186A/G, +655C/T) and SP-B (1580C/T). However, no statistically significant differences were observed in the allele and genotype frequencies of SP-B –18A/C, SP-D Met11ThrT/C, and SP-D Ala160ThrG/A. Importantly, serum SP-A and SP-B levels were reduced in RDS patients carrying SP-A (+186A/G, +655C/T) and SP-B (1580C/T) polymorphisms. AA genotype of +186A/G, SP-A level, and CC genotype of 1580C/T were independently correlated with increased RDS risk.
CONCLUSIONS: SP-A (+186A/G) and SP-B (1580C/T) polymorphisms are strongly associated with the risk of RDS in preterm infants. Notably, reduced serum SP-A levels were correlated with a high risk of RDS and may serve as novel biomarkers for RDS detection and monitoring.

Keywords: Gene Frequency - genetics, Case-Control Studies, Genetic Predisposition to Disease, Haplotypes - genetics, Infant, Newborn, Infant, Premature - metabolism, Logistic Models, Polymorphism, Single Nucleotide - genetics, Pulmonary Surfactant-Associated Protein A - genetics, Pulmonary Surfactant-Associated Protein B - genetics, Pulmonary Surfactant-Associated Protein D - genetics, Respiratory Distress Syndrome, Newborn - genetics, Risk Factors



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