Parathyroid Hormone-Induced Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation and its Repair of Articular Cartilage Injury in Rabbits
Yushu Chen, Yi Chen, Shujiang Zhang, Xiufan Du, Bo Bai
Department of Orthopedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China (mainland)
Med Sci Monit Basic Res 2016; 22:132-145
Available online: 2016-11-16
We explored the effect of parathyroid hormone (PTH)-induced bone marrow stem cells (BMSCs) complexed with fibrin glue (FG) in the repair of articular cartilage injury in rabbits.
MATERIAL AND METHODS: Forty-eight rabbits randomized into four groups were subjected to articular surgery (cartilage loss). The PTH and non-PTH intervention groups included transplantation with PTH/BMSC/FG xenogeneic and BMSC/FG xenogeneic complexes, respectively, into the injured area. The injured group contained no transplant while the control group comprised rabbits without any articular injury. Samples were monitored for cartilage repair up to three months post-surgery. Immunohistochemistry as well as real-time fluorescent quantitative PCR and Western blot were used to analyze the expression of type II collagen and aggrecan in the repaired tissue.
RESULTS: At 12 weeks post-surgery, the loss of articular cartilage in the PTH group was fully repaired by hyaline tissue. Typical cartilage lacunae and intact subchondral bone were found. The boundary separating the surrounding normal cartilage tissue disappeared. The gross and International Cartilage Repair Society (ICRS) histological ranking of the repaired tissue was significantly higher in the PTH intervention group than in the non-PTH intervention and injury groups (p<0.05) without any significant difference compared to the control group (p>0.05). Type II collagen and aggrecan stained positive and the average optical density, relative mRNA expression and protein-integrated optical density in the PTH group were higher than in non-PTH and injured groups (p<0.05) but not significantly different from the control group (p>0.05).
CONCLUSIONS: PTH/BMSC/FG xenogeneic complexes effectively repaired the loss of cartilage in rabbit knee injury.
Keywords: Cartilage, Articular, Mesenchymal Stem Cell Transplantation, Parathyroid Hormone