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03 March 2017 : Laboratory Research  

Prolonged Integration Site Selection of a Lentiviral Vector in the Genome of Human Keratinocytes

Wei Qian1ABCDEF, Yong Wang1ABCDEF, Rui-fu Li1BCDF, Xin Zhou1BCF, Jing Liu1BCF, Dai-zhi Peng1ABCDEFG*

DOI: 10.12659/MSM.903094

Med Sci Monit 2017; 23:1116-1122

Abstract

BACKGROUND: Lentiviral vectors have been successfully used for human skin cell gene transfer studies. Defining the selection of integration sites for retroviral vectors in the host genome is crucial in risk assessment analysis of gene therapy. However, genome-wide analyses of lentiviral integration sites in human keratinocytes, especially after prolonged growth, are poorly understood.

MATERIAL AND METHODS: In this study, 874 unique lentiviral vector integration sites in human HaCaT keratinocytes after long-term culture were identified and analyzed with the online tool GTSG-QuickMap and SPSS software.

RESULTS: The data indicated that lentiviral vectors showed integration site preferences for genes and gene-rich regions.

CONCLUSIONS: This study will likely assist in determining the relative risks of the lentiviral vector system and in the design of a safe lentiviral vector system in the gene therapy of skin diseases.

Keywords: Keratinocytes, Lentivirus, Virus Integration

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Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416