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Protective Role of TNIP2 in Myocardial Injury Induced by Acute Pancreatitis and Its Mechanism

Hualei Xie, Maoyong Yang, Bo Zhang, Min Liu, Shumei Han

Department of Emergency Medicine, The Second People’s Hospital of Liaocheng affiliated to Taishan Medical College, Linqing, Shandong, China (mainland)

Med Sci Monit 2017; 23:5650-5656

DOI: 10.12659/MSM.904398

Available online:

Published: 2017-11-27

BACKGROUND: Aberrant regulation of nuclear factor-κB (NF-κB) and the signaling pathways that regulate its activity have been found to be involved in various pathologies, particularly cancers, as well as inflammatory and autoimmune diseases. Acute pancreatitis (AP) is a complex pathological process, depending on autodigestion caused by premature activation of zymogens. This study aimed to investigate the effect of high expression of TNIP2 gene on AP and AP-induced myocardial injury.
MATERIAL AND METHODS: To investigate the effect of TNIP2 on AP and AP-induced myocardial injury, we established an AP cell model and rat model. HE staining was applied for histological examination. ELISA was used to determine the level of pro-inflammatory cytokines (TNF-α and IL-6) and myocardial injury markers (LDH and CK-MB). QRT-PCR and Western blot analysis were performed to determine the mRNA and protein level of related genes, respectively.
RESULTS: We found that the protein level of TNIP2 was relatively higher in the normal AR42J cells. At 4 h after stimulating with cerulein, the protein level of TNIP2 decreased, reached a minimum at 8 h, and then gradually increased. We also found that TNIP2 was correlated with the activation of NF-κB in cerulein-stimulated AR42J cells, and TNIP2 over-expression inhibited the inflammatory response caused by cerulein. Moreover, our results suggest that TNIP2 over-expression relieved the cerulein-triggered inflammatory response and AP-induced myocardial injury in mice.
CONCLUSIONS: TNIP2 was shown to exert a protective effect on AP and AP-induced myocardial injury.

Keywords: Heart Injuries, NF-kappa B, Pancreatitis