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05 April 2018 : Laboratory Research  

Protective Effects of Sinomenine on CFA-Induced Inflammatory Pain in Rats

Yan Yuan1ABCDEFG, Yongjun Zhang1BCD, Xiaofeng He1EF, Shengdeng Fan1ABCDEFG*

DOI: 10.12659/MSM.906726

Med Sci Monit 2018; 24: LBR2018-2024

Abstract

BACKGROUND: The purpose of this study was to investigate the effects of sinomenine (SIN) on CFA-induced inflammatory pain in rats, and to explore the underlying molecular mechanisms.

MATERIAL AND METHODS: To determine the potential influences of SIN in the pathogenesis of inflammatory pain, an inflammatory pain (IP) mouse model was established and rats were treated with SIN (30 mg/kg). Behavioral tests were used to assess the MWT and TWL of the rats. ELISA assay was used to detect the level of inflammation cytokines. Western blotting and qRT-PCR were carried out to measure the related protein and mRNA expression level, respectively.

RESULTS: We found that the MWT and TWL of the CFA-treated rats were markedly lower than that of the control rats, and they were significantly increased by SIN administration. The results suggest that IP rats had higher levels of TNF-α, IL-1β and IL-6 compared with the control rats. SIN administration decreased the levels of TNF-α, IL-1β, and IL-6. In addition, we found that p-p65 and p-p38 expression notably decreased after SIN treatment in IP rats. Moreover, the results showed that SIN inhibited Cox-2 and PGE2 expression in IP rats.

CONCLUSIONS: The data indicate that SIN had a protective role in inflammatory pain through repressing inflammatory mediators via preventing the p38MAPK-NF-κB pathway.

Keywords: Inflammation Mediators, NF-kappa B, p38 Mitogen-Activated Protein Kinases, Pain, Sinomenium

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Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416