Logo Medical Science Monitor Basic Research

Call: 1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Contact Us

Logo Medical Science Monitor Basic Research Logo Medical Science Monitor Basic Research Logo Medical Science Monitor Basic Research

13 March 2018 : Original article  

Effects of the Phosphodiesterase-5 (PDE-5) Inhibitors, Avanafil and Zaprinast, on Bone Remodeling and Oxidative Damage in a Rat Model of Glucocorticoid-Induced Osteoporosis

Zübeyir HuyutABCDEFG, Nuri BakanACD, Serkan YıldırımBCD, Hamit Hakan ALPACD

DOI: 10.12659/MSMBR.908504

Med Sci Monit Basic Res 2018; 24:47-58

Abstract

BACKGROUND: The aim of this study was to evaluate the effects of the phosphodiesterase-5 (PDE-5) inhibitors, zaprinast and avanafil, on NO signalling pathway, bone mineral density (BMD), epiphyseal bone width, bone marrow angiogenesis, and parameters of oxidative stress in a rat model of glucocorticoid-induced osteoporosis (GIOP).

MATERIAL AND METHODS: Twenty-four 8-month-old male rats in four groups were given a single daily treatment during a 30-day period: an (untreated) control group (n=6): a dexamethasone-treated group (120 µ/kg) (n=6); a group treated with dexamethasone (120 µ/kg) and zaprinast (10 mg/kg) (n=6): and a group treated with dexamethasone (120 µ/kg) and avanafil (10 mg/kg) (n=6). Rat whole body bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DEXA), and bone histology was performed. Also, selected oxidative stress parameters by HPLC method and the other biochemical parameters by ELISA method were measured.

RESULTS: The GIOP model rats treated with zaprinast and avanafil showed a significant increase in NO, cyclic guanosine monophosphate (cGMP), and protein kinase G (PKG) (NO/cGMP/PKG) signaling-pathway components, and in C-terminal telopeptide of type I collagen (CTX-1), bone marrow angiogenesis, BMD, and epiphyseal bone width, compared with the (untreated) control rats (p<0.05). Levels of pyridinoline (PD) and deoxypyridinoline (DPD) were significantly reduced in the dexamethasone + zaprinast, and dexamethasone + avanafil treatment groups (p<0.05). Malondialdehyde (MDA), ubiquinone-10 (CoQ10), ubiquinol CoQ10 (CoQ10H), and 8-hydroxy-2’-deoxyguanosine (8-OHdG) were significantly increased in the dexamethasone-treated group, compared with the (untreated) controls (p<0.05).

CONCLUSIONS: In the GIOP rat model, markers of oxidative stress and bone atrophy were significantly reduced by treatment with the PDE-5 inhibitors, zaprinast and avanafil.

Keywords: Bone Density, Dexamethasone, Osteoporosis, Phosphodiesterase 5 Inhibitors

Add Comment 0 Comments

Most Viewed Current Articles

30 Oct 2023 : Original article   5,615

Exploring the Impact of the COVID-19 Pandemic on Academic Burnout Among Nursing College Students in China: ...

DOI :10.12659/MSMBR.940997

Med Sci Monit Basic Res 2023; 29:e940997

22 Mar 2023 : Clinical Research   4,509

A Questionnaire-Based Study to Compare the Psychological Effects of 6 Weeks of Exercise in 123 Chinese Coll...

DOI :10.12659/MSMBR.939096

Med Sci Monit Basic Res 2023; 29:e939096

10 Jan 2023 : Clinical Research   3,531

Prevalence and Associated Factors of Depression Among Frontline Nurses in Wuhan 6 Months After the Outbreak...

DOI :10.12659/MSMBR.938633

Med Sci Monit Basic Res 2023; 29:e938633

06 Nov 2023 : Original article   3,125

Urinary Klotho Excretion: A Key Regulator of Sodium Homeostasis in Chronic Kidney Disease Stage 2-4

DOI :10.12659/MSMBR.942097

Med Sci Monit Basic Res 2023; 29:e942097

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416