Logo Medical Science Monitor Basic Research

Call: 1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Contact Us

Logo Medical Science Monitor Basic Research Logo Medical Science Monitor Basic Research Logo Medical Science Monitor Basic Research

27 August 2018 : Clinical Research  

MicroRNA-495 Confers Increased Sensitivity to Chemotherapeutic Agents in Gastric Cancer via the Mammalian Target of Rapamycin (mTOR) Signaling Pathway by Interacting with Human Epidermal Growth Factor Receptor 2 (ERBB2)

Na Li1BC, Mei Han1AB, Ning Zhou1BC, Yong Tang1DE, Xu-Shan Tang1DE*

DOI: 10.12659/MSM.909458

Med Sci Monit 2018; 24: CLR5960-5972

Abstract

BACKGROUND: In recent years, the incidence of gastric cancer (GC) presents is increasing and plagues people worldwide. Emerging evidence shows that microRNAs (miRs) may be involved in the pathogenesis of GC. Thus, this paper aims to explore the mediatory role of miR-495 in GC chemosensitivity, and the mechanism by which it affected the biological behaviors of GC cell via the mTOR signaling pathway.

MATERIAL AND METHODS: After GC and paracancerous tissue collection, the positive rate of ERBB2 and mTOR was evaluated by immunohistochemistry. Subsequently, the expression of miR-495, ERBB2 and mTOR was determined by RT-qPCR and western blot analysis. Next, the targeting relationship between miR-495 and ERBB2 was confirmed by dual-luciferase reporter gene assay. In addition, chemosensitivity and proliferation were detected by MTT assay, and apoptosis by flow cytometry.

RESULTS: We firstly observed that higher positive rate of ERBB2 and mTOR as well as decreased expression of miR-495 were found in GC tissues. Additionally, ERBB2 is the target gene of miR-495. Furthermore, it was determined that overexpressed miR-495 or silencing ERBB2 enhanced GC cells chemosensitivity and apoptosis, but inhibited GC cell proliferation. Besides, we found that the effect of miR-495 inhibition was lost when ERBB2 was suppressed.

CONCLUSIONS: The key findings of our study demonstrate that the miR-495 exerts promoting effects on GC chemosensitivity via the inactivation of mTOR signaling pathway by suppressing ERBB2. The study provides reliable reference for the application of miR-495 as a novel potential target in the chemotherapy of GC.

Keywords: Gastroenterology, Stomach Diseases

Comments

Most Viewed Current Articles

13 Apr 2020 : Original article  

Outcome of 24 Weeks of Combined Schroth and Pilates Exercises on Cobb Angle, Angle of Trunk Rotation, Chest...

DOI :10.12659/MSMBR.920449

Med Sci Monit Basic Res 2020; 26:e920449

11 May 2020 : Original article  

Analysis of Psychological and Sleep Status and Exercise Rehabilitation of Front-Line Clinical Staff in the ...

DOI :10.12659/MSMBR.924085

Med Sci Monit Basic Res 2020; 26:e924085

05 Jan 2021 : Review article  

A Southeast Asian Perspective on the COVID-19 Pandemic: Hemoglobin E (HbE)-Trait Confers Resistance Against...

DOI :10.12659/MSMBR.929207

Med Sci Monit Basic Res 2021; 27:e929207

10 Aug 2020 : Clinical Research  

Effects of Cognitive Task Training on Dynamic Balance and Gait of Patients with Stroke: A Preliminary Rando...

DOI :10.12659/MSMBR.925264

Med Sci Monit Basic Res 2020; 26:e925264

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416