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18 February 2019 : Original article  

miR-193a-3p Promotes Radio-Resistance of Nasopharyngeal Cancer Cells by Targeting SRSF2 Gene and Hypoxia Signaling Pathway

Lingsuo Kong12ABE, Qing Wei2C, Xianwen Hu3D, Lanren Chen2F, Juan Li14AG*

DOI: 10.12659/MSMBR.914572

Med Sci Monit Basic Res 2019; 25:53-62

Abstract

BACKGROUND: Radio-resistance is an important barrier in nasopharyngeal carcinoma treatment. MicroRNAs are gene expression core regulators in various biological procedures containing cancer radio-resistance. Nevertheless, the clinical association between nasopharyngeal carcinoma and miR-193a-3p/SRSF2 remains unclear.

MATERIAL AND METHODS: We examined the miR-193a-3p level in radio-sensitive CNE-2 and radio-resistant CNE-1 NPC cell lines, and, based on a literature review, predicted SRSF2 to be the target gene of miR-193a-3p. We explored the expression of SRSF2 at protein and mRNA levels by transfecting either miR-193a-3p-mimic or antagomiR. Finally, we performed signaling pathway analysis to assess the possible role of miR-193a-3p/SRSF2 in signaling pathways.

RESULTS: miR-193a-3p promotes NPC radio-resistance, and the SRSF2 gene is the direct target for miR-193a-3p in NPC, and thus is negatively correlated with NPC radio-resistance. The hypoxia signaling pathway activity is strongly affected, and it is possible to use the downstream activity of the SRSF2 gene to show the effect of miR-193a-3p on radio-resistance in NPC cells.

CONCLUSIONS: miR-193a-3p mediates promotion of NPC radio-resistance.

Keywords: Head and Neck Neoplasms, Nasopharyngeal Diseases, Nasopharyngeal Neoplasms, Apoptosis, Cell Movement, Cell Proliferation, Gene Expression Regulation, Neoplastic, Gene Targeting, hypoxia, MicroRNAs, Radiation Tolerance, Serine-Arginine Splicing Factors, Signal Transduction, Xenograft Model Antitumor Assays

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Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416