H-Index
10
Scimago Lab
powered by Scopus
eISSN: 2325-4416
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Logo

MSMbanner
AmJCaseRep

Annals
ISI-Home

Chemical Composition and Biological Activities of Lebanese Pentapera Plant

Hany Kallassy, Hussein Fayyad-Kazan, Rawan Makki, Monya Kaeen, Amer Sakr, Osama Alyamani, Rim El Dirani, Eva Hamade, Mohammad Fayyad-Kazan, Bassam Badran

Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, Lebanese University, Hadath, Beirut, Lebanon

Med Sci Monit Basic Res 2019; 25:88-99

DOI: 10.12659/MSMBR.914741

Available online:

Published: 2019-03-14


#914741

BACKGROUND: Due to their chemical constituents and biological properties, plants have long been used to control life-threatening diseases. The flora of Lebanon includes many plants that have already been demonstrated to have medicinal value, and other species, such as Pentapera sicula libanotica, that are yet to be characterized. The present study characterized the chemical composition, anti-oxidant, anti-inflammatory, and anti-proliferative potential of aqueous, ethanol, and methanol extracts derived from the leaves of the Lebanese Pentapera plant.
MATERIAL AND METHODS: High-performance liquid chromatography (HPLC) was used to determine the chemical composition. Gas chromatography (GC) coupled with mass spectrometry (MS) was applied to determine the content of essential oil. DPPH radical scavenging assay was performed to evaluate the anti-oxidant potential. The anti-inflammatory potential was assessed using quantitative real-time PCR (qRT-PCR) by measuring TNF-alpha, IL-6, and CCL4 mRNA levels, and we assessed Cox-2 and iNOS proteins levels using Western blot (WB) analysis. MTT assay was carried out to determine the anti-proliferative potential.
RESULTS: We identified, mainly in the alcoholic (methanol and ethanol) extracts, distinct bioactive compounds with pharmacological relevance. In parallel, with their phytochemical content, these 2 extracts showed significant anti-oxidant, anti-inflammatory and anti-proliferative capacities.
CONCLUSIONS: Pentapera sicula libanotica appears to be a promising pharmacological tool.

Keywords: Anti-Inflammatory Agents, Antioxidants, Cell Survival



Back