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27 August 2019 : Clinical Research  

Expression of KIF18A Is Associated with Increased Tumor Stage and Cell Proliferation in Prostate Cancer

Hua Zhang1AB, Tianyu Shen23BC, Zheng Zhang2CD, Yang Li2F, Zhongjie Pan1AG*

DOI: 10.12659/MSM.917352

Med Sci Monit 2019; 25:6418-6428

Abstract

BACKGROUND: The role of KIF18A in tumorigenesis and tumor development has been well studied in several cancers, but not in prostate cancer. In this study, we investigated the potential prognostic utility of KIF18A and its role in prostate cancer progression.

MATERIAL AND METHODS: We collected prostate cancer and paracancerous tissue samples from the same patient. Immunohistochemical staining was performed to investigate the KIF18A expression levels in the clinical sample. The Cancer Genome Atlas (TCGA) database was analyzed via a bioinformatics approach to gain insight into the relationship between KIF18A expression and prognosis. We examined the effect of KIF18A knockdown on PC-3 cell proliferation via colony formation and MTT assays. Flow cytometry was used to assess the effect of KIF18A knockdown on PC-3 cell apoptosis. Transwell invasion assay was performed to assess whether KIF18A affects the invasion ability of PC-3 cells.

RESULTS: The KIF18A protein level was higher in PCa tissue than in paracancerous tissue. The In addition, upregulated KIF18A suggested a poor tumor stage and prognosis for prostate cancer patients. Our in vitro experiments demonstrated that KIF18A knockdown in PC-3 cells significantly inhibited proliferation and metastasis.

CONCLUSIONS: High KIF18A expression in prostate cancer patients predicts a poor prognosis. KIF18A knockdown inhibits prostate cell proliferation and metastasis. Therefore, this study confirms the usefulness of KIF18A as an oncological prognostic indicator and a potential therapeutic target for prostate cancer.

Keywords: Cell Proliferation, Lymphatic Metastasis, Prognosis, Prostatic Neoplasms, Survival Analysis, Aged, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Kinesins, Middle Aged, Neoplasm Grading, Neoplasm Metastasis, Proportional Hazards Models, Prostatectomy, RNA, Messenger, Up-Regulation

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Medical Science Monitor Basic Research eISSN: 2325-4416
Medical Science Monitor Basic Research eISSN: 2325-4416