Vitamin D3 Is Transformed into 1,25(OH)2D3 by Triggering CYP3A11(CYP3A4) Activity and Hydrolyzing Midazolam
Hanfei Zhu, Ruihan Wu, Zijun Gu, Minghui Ji, Qin Xu
School of Nursing, Nanjing Medical University, Nanjing, Jiangsu, China (mainland)
Med Sci Monit 2019; 25:9159-9166
Vitamin D3 (VD3) is a commonly used supplement in clinical practice. Cytochrome P450 3A11 (CYP3A11) is the most important monomeric enzyme involved in metabolism of drugs. This study aimed to investigate effects of vitamin D3 (VD3) on CYP3A11 activity.
MATERIAL AND METHODS: Forty male Sprague-Dawley (SD) rats were randomly divided a Control group (peanut oil 0.1 ml/kg/d), a Low-VD3 group (100 IU/kg/d), a Medium-VD3 group (400 IU/kg/d), and a High-VD3 (1600 IU/kg/d) group. Blood samples were collected from the jugular vein after midazolam (MDZ) administration. CYP3A11 expressions in liver and colon were detected by Western blotting and immunohistochemistry (IHC) assay. The concentration of serum 25(OH)D₃ and serum 1,25(OH)₂D₃ were evaluated using ELISA. Effects of different dosages of vitamin D3 on metabolism of MDZ were evaluated using high-performance liquid chromatography (HPLC).
RESULTS: Vitamin D3 significantly enhanced serum 25(OH)D₃ and 1,25(OH)2D₃ levels in rats compared to Control rats (p<0.05). Expressions of hepatic CYP3A11 were more than 10-fold higher in rats treated with vitamin D3 compared to Control rats (p<0.05). Expressions of colon CYP3A11 were 5-fold higher than in Control rats (p<0.05). CYP3A11 expressions in vitamin D3-treated groups were significantly higher compared to the Control group (p<0.05). MDZ levels were significantly higher in Vitamin D3-treated rats compared to that in Control rats (p<0.05). Concentrations of serum MDZ at every sampling point were remarkably lower in the vitamin D3-treated rats than in Control rats (p<0.05).
CONCLUSIONS: Vitamin D3 was transformed into 1,25(OH)₂D₃ by triggering CYP3A11 and CYP3A11 activity and by hydrolyzing MDZ.
Keywords: 24,25-Dihydroxyvitamin D 3, Basal Metabolism, Midazolam