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Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis

Zheng Chen, Zhen Zhang, Dongbo Zhao, Wei Feng, Fanlai Meng, Shihui Han, Bin Lin, Xin Shi

Department of General Surgery, The Affiliated Suqian Hospital of Xuzhou Medical University, Suqian People’s Hospital of Nanjing Drum Tower Hospital Group, Suqian, Jiangsu, China (mainland)

Med Sci Monit 2019; 25:9618-9629

DOI: 10.12659/MSM.918230

Available online:

Published: 2019-12-16

BACKGROUND: The aim of this study was to investigate the effects and mechanisms of long noncoding (lnc) RNA FOXD2-AS1 in hepatocellular carcinoma development.
MATERIAL AND METHODS: Collecting the 3 pairs of adjacent and hepatocellular carcinoma tissue and analysis by gene chip. Evaluating the FOXD2-AS1 expression by in situ hybridization assay. Evaluating the FOXD2-AS1 to Bel-7402 biological activity in vitro study by Cell Counting Kit-8, flow cytometry, Transwell and wound healing assay and correlation between miR-185 by dual-luciferase reporter assay. The relative proteins expressions were evaluated by western blot assay.
RESULTS: FOXD2-AS1 was significantly upregulation in hepatocellular carcinoma tissues. FOXD2-AS1 knockdown suppressed Bel-7401 cell biological activities (proliferation, invasion, and migration) with miR-185 overexpression and AKT depressing in cell expression.
CONCLUSIONS: LncRNA FOXD2-AS1 promoted hepatocellular carcinoma development by regulation miR-185/AKT axis.

Keywords: Biological Transport, Active, MicroRNAs, Proto-Oncogene Proteins c-akt, RNA, Long Noncoding