Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

19 February 2020 : Laboratory Research  

miRNA-411 Regulates Chondrocyte Autophagy in Osteoarthritis by Targeting Hypoxia-Inducible Factor 1 alpha (HIF-1α)

Fei Yang1ABCDEF, Rong Huang2ABCD, Hui Ma1BC, Xiaowei Zhao1EF*, Guodong Wang1ACEG

DOI: 10.12659/MSM.921155

Med Sci Monit 2020; 26:e921155

Abstract

BACKGROUND: Osteoarthritis (OA) is the most common joint disease and is characterized by the progressive degeneration of articular cartilage. The molecular basis of OA involves various factors and has not been fully clarified. Autophagy is a conserved catabolic process that involves cellular degradation through the lysosomal machinery.

MATERIAL AND METHODS: We found that miRNA-411 regulates chondrocyte autophagy in OA by targeting hypoxia-inducible factor 1 alpha (HIF-1α) and identified the related molecular mechanism. OA condition in chondrocyte C28/I2 cells was induced by treatment with interleukin 1 beta (IL-1β). The protein expressions of LC3, p62, HIF-1α, ULK-1, and Beclin-1 were assessed by Western blot analysis, and LC3 expression was assessed by immunofluorescence.

RESULTS: TargetScan analysis showed that HIF-1α mRNA is directly targeted by miR-411, which was confirmed by luciferase reporter assay. miR-411 mimic decreased HIF-1α levels in chondrocytes while miR-411 inhibitor increased HIF-1α levels in chondrocytes. Furthermore, expression of LC3, ULK-1, P62, and Beclin-1 in chondrocytes was induced by miR-411 inhibitor and was downregulated by miR-411 mimics. In addition, miR-411 mimics reduced the expression level of LC3, as determined by immunofluorescence analysis.

CONCLUSIONS: Our results demonstrate that miR-411 promotes chondrocyte autophagy by targeting HIF-1α, suggesting that regulating HIF-1α by miR-411 might be a therapeutic strategy for OA.

Keywords: Hypoxia-Inducible Factor-Proline Dioxygenases, Osteoarthritis, Base Sequence, Cell Line, Chondrocytes, Hypoxia-Inducible Factor 1, alpha Subunit

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750