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Identification of Differentially Expressed Proteins in Serum of Obese Patients by Isobaric Tags for Relative and Absolute Quantification (iTRAQ)-Coupled 2D LC-MS

Ying Zhang, Zhong Chen, Yue He, Jianxia Wang, Minhui Jiang, Jianjuan Xu, Minghua Chen, Yaling Feng

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland)

Med Sci Monit 2020; 26:e924882

DOI: 10.12659/MSM.924882

Available online: 2020-06-04

Published: 2020-08-02

BACKGROUND: The aim of this study was to identify the differentially expressed proteins of obese patients compared with normal participants and to provide a potential target for future investigation of obesity.
MATERIAL AND METHODS: We enrolled 10 obese male adults and 10 matched normal subjects. Serum samples were collected to get total protein extraction, denaturation, deoxidation, and enzymatic hydrolysis. Differentially expressed proteins were distinguished with mass spectrometry after samples were labeled with iTRAQ.
RESULTS: A total of 9622 differentially expressed peptides were identified, corresponding to 733 proteins; 118 proteins of these showed significant differential expression, with 15 upregulated and 103 downregulated.
CONCLUSIONS: iTRAQ is an effective technique to identify differentially expressed proteins in obese patients. The development of obesity is correlated with a series of complex elements and mutual effects. The proteins identified in this study may provide novel directions and targets for future pathological studies of obesity.

Keywords: 14-3-3 Proteins, Obesity, Abdominal, Serum Albumin, Bovine