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Disinfection of Infected Artificial Dental Periapical Lesions with Diode Laser: An In Vitro Study

Agron Bytyqi, Xhevdet Aliu, Merita Barani, David Stubljar, Tomislav Jukic, Andrej Starc, Sokol Krasniqi

College of Medical Sciences – Rezonanca, Pristina, Kosovo

Med Sci Monit Basic Res 2021; 27:e932492

DOI: 10.12659/MSMBR.932492

Available online: 2021-07-27

Published: 2021-07-27


BACKGROUND: Periapical lesions are primarily caused by infections in the root canals. The objective of this study was to assess the antibacterial effectiveness of diode laser during root canal treatment in artificial models of infected periapical lesions.
MATERIAL AND METHODS: One hundred twenty-two extracted premolar single-rooted teeth were inserted into methyl methacrylate artificial models of periapical lesions, and bacterial solutions of Enterococcus faecalis (ATCC 29212) and Streptococcus mitis (ATCC 49456) were then applied to the models. The respective diameters of lesions in the artificial models represented 3 different subgroups based on lesion size. The laser protocol used for endodontic disinfection had a power output of 1.5 W and a wavelength of 810 nm. The impact on cell viability was evaluated by flow cytometry.
RESULTS: Disinfection with laser did not differ between microorganisms (P=0.137), and laser irradiation with a longer duration had better disinfecting action for both microorganisms (P<0.001). Compared with larger lesions, smaller lesions had a higher percentage of dead cells for both microorganisms (P<0.001). The percentage of dead cells in the treatment groups was significantly higher than in the control group (P<0.001).
CONCLUSIONS: Laser treatment had a poor, almost negligible effect on elimination of bacterial cells in large periapical lesions. Application of a laser might serve as an adjuvant method to standard irrigation with sodium hypochlorite.

Keywords: Enterococcus faecalis, Lasers, Periapical Diseases, Streptococcus mitis, Root Canal Therapy