Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 November 1996

Insulin dependent diabetes mellitus (type 1) analysis of HLA DQA1(52) and HLA DQB1(57) codons

Henryk W Witas, Jerzy Bodalski, Krystyna Jędrychowska-Dańska, Marcin Różalski, Wojciech Mlynarski, Dorota Cedzyńska, Ewa Brzeziańska

Med Sci Monit 1996; 2(6): CR734-739 :: ID: 500135

Abstract

Allele-specific amplification PCR method (ASA-PCR) allows to genotype possible codons at HLA-DQA152, and HLA-DQB157, locations which in turn allows the definition of expressed amino acid. The analysis of codon variants frequency was performed in insulin dependent diabetes mellitus (IDDM) children (n=50) and control subjects (n=50) of Polish origin. Aliphatic and hydrophobic amino acids residues (phenotypes: Val/Ala and Ala/Ala) were found to occur significantly more frequently at DQ&β57, in IDDM children (74%) than in control subjects (18%); RR=12.96. The dominant protective effect of DQβAsp57, is suggested. Codons for Asp/Asp were not found in IDDM children while they were exhibited by 28% of controls (RR=0.025). Simultaneous typing of both codons revealed that DQαArg52+/DQβAsp57-, accompany the IDDM phenotype much more frequently than the physiological one (68% v. 22% RR=7.53). The analysis of a number of susceptibility (αArg52+,/βAsp57-,) heterodimers which can occur in one child revealed that the exclusive expression of such dimers (100%) and presence of half of them (50%) was more frequently in the IDDM group (RR= 4.47 and 2.57, respectively). The lack of αArg52+,/βAsp57-, dimers or their involvement only at rate of 1/4 (25%) is characteristic of the control group (RR=0.173 calculated for both cases together). The presented results are the first qualitative and quantitative analysis of studied polymorphism of genes coding the dimeric protein which presents antigen on the cell surface. This preliminary studies contribute to a detailed analysis of allelic variants in the Polish population which is necessary in the evaluation of children belonging to the group a high risk for IDDM. Supported by grant KBN No. 4 P05E 053 08

Keywords: insulin dependent diabetes mellitus, IDDM, HLA, DNA amplification, Genetics

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

26 Feb 2024 : Clinical Research  

Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...

Med Sci Monit In Press; DOI: 10.12659/MSM.942509  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750