Clinical Features of 4 Novel NOTCH3 Mutations of Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy in China

Weiwei Qin; Zhixia Ren; Mingrong Xia; Miaomiao Yang; Yingying Shi; Yue Huang; Xiangqian Guo; Jiewen Zhang

doi:10.12659/MSMBR.918830

26 September 2019 : Original article

Clinical Features of 4 Novel NOTCH3 Mutations of Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy in China

Weiwei Qin^{ABCDEF 1}, Zhixia Ren^{ABCDEF 1}, Mingrong Xia^{ABCD 1}, Miaomiao Yang^{ABCD 1,2}, Yingying Shi^{ABCD 1}, Yue Huang^{ADEF 1}, Xiangqian Guo^{CEF 3}, Jiewen Zhang^{ACDEFG 1*}

DOI: 10.12659/MSMBR.918830

Med Sci Monit Basic Res 2019; 25:199-209

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Supplementary Table 1. Primers for amplification of NOTCH3 exons.

Exons	Primer sequence (from 5′ to 3′)	Target (bp)	Annealing temperature (°C)
Exon 2	AGACCTCCCTGTAACACTCAGCACC	616	55
Exon 2	AGAGCCCCTGCTCAGTTTCCAAT	616	55
Exon 3	GCAAATGTGTGTTTGCTGCT	482	55
Exon 3	CACTCATCCACGTCGCTTC	482	55
Exon 4	TCCTAAACTCACCCTGTCCTG	559	60
Exon 4	TTATAGGTGTTGACGCCATCC	559	60
Exon 5	GGCGACCTCACTTACGACTG	454	56
Exon 5	GTCCAGCCATTGACACACAC	454	56
Exon 6	CTGGACTGCTGCATCTGTGT	283	55
Exon 6	TGCAGAGAAAACGGCCACTC	283	55
Exon 7	TACTCAAGGGGTGTGGGC	319	55
Exon 7	GATGGAGTGCGATCGGTGT	319	55
Exon 8	TGTGGACGAGTGCTCTATCG	400	57
Exon 8	CCCACTTACACCCCATTCTG	400	57
Exon 9	GAGTGTAGAGGTGGGGACGA	407	55
Exon 9	TGGGTGGAAAAACCCATTTA	407	55
Exon 10	CAGGGTGGGAACCTGTAAAA	481	56
Exon 10	ACTTTGGCTCCACACGTAGC	481	56
Exon 11	GAGCTGAACCAGGATTGGTC	453	60
Exon 11	TTCACTTCGCAGTTCACACC	453	60
Exon 12	AAATGCCTAGACCTGGTGGA	385	55
Exon 12	CGTTGGACAAGAGTCTGCAA	385	55
Exon 13	GGTGTGCTAAGTGGGGTCAC	485	57
Exon 13	CAGTGGAAACCCATTCCATC	485	57
Exon 14	AGGGAGGTTCCTGTGTGGAT	499	56
Exon 14	AGCTCCTGGAGGGAAATGAT	499	56
Exon 15	CTCTCTCCTCCCGCTATGTT	578	58
Exon 15	GCCGTCTCCCTGTCTCAGTA	578	58
Exon 16	TGACAGCACGGCTTATTTTG	476	55
Exon 16	CCAGCTCATTCCCTAACTCG	476	55
Exon 17	CTGTCCTTGTCCTGTTCCAA	659	60
Exon 17	CAGTCATCAGAGTCCGCAGT	659	60
Exon 18	CTTGCCGAGATAAGGGTCAG	450	55
Exon 18	AGGGGAAGCACTCAGAGTCA	450	55
Exon 19	GGCACAGTTTCTCCCAGACTA	455	51
Exon 19	CCTTCAACGCTCACAGCAG	455	51
Exon 20	GACTCATTCCACCAAGGATGTT	430	51
Exon 20	CACCCACAGATACACCAAGAGT	430	51
Exon 21–23	ACCTACTGGTAGTGCGCTGAACATC	937	55
Exon 21–23	TGTCAGCATTTTCCAGAAACTCCCT	937	55
Exon 24	GGAGCGGTTGGGTGGGACA	982	55
Exon 24	GGCAGACAAACGGGGCAGAG	982	55

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Clinical Features of 4 Novel NOTCH3 Mutations of Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy in China

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