13 November 2013 : Original article
Leptin increases mitotic index and regeneration ratio in hepatectomized rats
Murat CilekarABCDEF, Onur UysalABCF, Cengiz BalBCDEF, Serkan TurelCDEF, Sezgin YılmazCDEFDOI: 10.12659/MSMBR.889591
Med Sci Monit Basic Res 2013; 19:279-284
Abstract
BACKGROUND: The aim of this study was to evaluate the potential effect of intraperitoneal administration of leptin on the hepatic regeneration and the mitotic index.
MATERIAL AND METHODS: 56 Sprague-Dawley rats were divided into 7 groups each containing 8 rats. Group 1 was evaluated as the sham group and no surgical procedure was performed on animals. The rats in groups 2, 3, and 4 (named C24, C48, C72, respectively) were given intraperitoneal injection of 2 ml/kg normal saline 60 minutes before the surgical procedure consisting of laparotomy and 70% hepatectomy. These groups were used as controls at 24, 48, and 72 hours. The rats in groups 5, 6, and 7 (named L24, L48, and L72, respectively) were given intraperitoneal injection of 20 µg/kg doses of recombinant mouse leptin 60 minutes before the same surgical procedure. These groups were evaluated as the experiment groups at 24, 48, and 72 hours. Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI). The weights of the remaining livers were also noted.
RESULTS: The values of AST and ALT were higher in the groups that were administered leptin and they had significantly higher mitotic index than the other groups. Leptin also significantly increased the regeneration ratio as compared to the control group. The weights of the remaining livers were also higher in the leptin groups.
CONCLUSIONS: Intraperitoneal administration of leptin was observed to increase liver regeneration and mitotic rate in 70% hepatectomized rats.
Keywords: Alanine Transaminase - metabolism, Aspartate Aminotransferases - metabolism, Hepatectomy, Injections, Intraperitoneal, Leptin - pharmacology, Liver - surgery, Liver Regeneration - drug effects, Mitotic Index, Organ Size - drug effects
Most Viewed Current Articles
15 Jun 2022 : Clinical Research
Evaluation of Apical Leakage After Root Canal Obturation with Glass Ionomer, Resin, and Zinc Oxide Eugenol ...DOI :10.12659/MSMBR.936675
Med Sci Monit Basic Res 2022; 28:e936675
07 Jul 2022 : Laboratory Research
Cytotoxicity, Apoptosis, Migration Inhibition, and Autophagy-Induced by Crude Ricin from Ricinus communis S...DOI :10.12659/MSMBR.936683
Med Sci Monit Basic Res 2022; 28:e936683
01 Jun 2022 : Laboratory Research
Comparison of Sealing Abilities Among Zinc Oxide Eugenol Root-Canal Filling Cement, Antibacterial Biocerami...DOI :10.12659/MSMBR.936319
Med Sci Monit Basic Res 2022; 28:e936319
08 Dec 2022 : Original article
Use of Estimated Glomerular Filtration Rate and Urine Albumin-to-Creatinine Ratio Based on KDIGO 2012 Guide...DOI :10.12659/MSMBR.938176
Med Sci Monit Basic Res 2022; 28:e938176