Roles of Long Non-Coding RNA CCAT2 in Cervical Cancer Cell Growth and Apoptosis

Le Wu; Lixu Jin; Wenmiao Zhang; Lifang Zhang

doi:10.12659/MSM.897754

17 March 2016 : Laboratory Research

Roles of Long Non-Coding RNA CCAT2 in Cervical Cancer Cell Growth and Apoptosis

Le Wu^AE, Lixu Jin^BD, Wenmiao Zhang^CE, Lifang Zhang^ACE

DOI: 10.12659/MSM.897754

Med Sci Monit 2016; 22:875-879

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Abstract

BACKGROUND: This study aimed to analyze the potential function of lncRNA CCAT2 in cervical cancer cell proliferation and apoptosis.

MATERIAL AND METHODS: Expression level of CCAT2 in cervical cancer cell lines (HeLa, CaSki, and SiHa) was detected by quantitative real-time PCR. CCAT2 knockdown was established by transfecting siRNA into human cervical cancer cells. Its effects on cell proliferation were studied using cell-counting kit-8 assay. The effect of CCAT2 on cervical cancer cells cycle and apoptosis was assessed by flow cytometry assay.

RESULTS: CCK8 assay showed that CCAT2 knockdown inhibited cell proliferation in HeLa, CaSki, and SiHa cells. The flow cytometry confirmed the results that knockdown of CCAT2 could induce cervical cancer cells cycle G1 phase arrestment and trigger the cells apoptosis.

CONCLUSIONS: LncRNA CCAT2 promoted the proliferation and survival of cervical cancer cells.

Keywords: Apoptosis - genetics, Real-Time Polymerase Chain Reaction, Uterine Cervical Neoplasms - pathology

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