17 April 2018 : Laboratory Research
Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma
Bin-Bin Yu12ABCDEFG, Guo-Xiang Lin12ABDEFG, Ling Li123EF, Song Qu123EF, Zhong-Guo Liang12BCD, Kai-Hua Chen12CDE, Lei Zhou12CD, Qi-Teng Lu12CD, Yong-Chu Sun12CD, Xiao-Dong Zhu1234ADEFG*DOI: 10.12659/MSM.909832
Med Sci Monit 2018; 24: LBR2317-2329
Abstract
BACKGROUND: The purpose of this study was to determine whether cofilin-2 could serve as a protein marker for predicting radiotherapy response and as a potential therapeutic target in nasopharyngeal carcinoma (NPC).
MATERIAL AND METHODS: Cofilin-2 protein levels in serum and tissue samples from patients with NPC were assessed by sandwich ELISA and IHC. In vitro, cofilin-2 levels in CNE-2R cells were significantly higher than those of CNE-2 cells. Meanwhile, CNE-2R cells were silenced for cofilin-2 to obtain a stable cofilin-2-RNAi-LV3 cell line. Then, cell proliferation, radiosensitivity, invasion and migration abilities, cell cycle, and apoptosis were evaluated by Cell Counting Kit 8 assay (CCK-8), flow cytometry (FCM), clone formation assay, and in vitro.
RESULTS: The secreted levels of the cofilin-2 protein in radioresistant NPC patients were significantly higher than those of radiosensitive cases. After cofilin-2 knockdown in nasopharyngeal carcinoma CNE-2R cells, proliferation was decreased, while apoptosis and radiosensitivity were enhanced; cell cycle distribution was altered, and the transplanted tumors in nude mice grew significantly less.
CONCLUSIONS: Overall, our findings suggest that cofilin-2 acts as a marker for predicting radiotherapy response and is a potential therapeutic target in nasopharyngeal carcinoma.
Keywords: Biological Markers, Cofilin 2, Gene Targeting, Radiation Oncology
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