05 May 2022 : Laboratory Research
Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16-F10 Cells
Eva Krishna Sutedja1ABDEF, Daniar Amarassaphira2ABF, Hanna Goenawan34BCEF, Yuni Susanti Pratiwi34BDE, Nova Sylviana34BDF, Budi Setiabudiawan5ABDEF, Oki Suwarsa1BDEF, Raden Tina Dewi Judistiani6CEF, Unang Supratman7BEF, Ronny Lesmana34ABCDEFG*DOI: 10.12659/MSMBR.935139
Med Sci Monit Basic Res 2022; 28:e935139
Figure 3 The effect of calcitriol on the gene expression of apoptosis-related proteins using real-time PCR in B16–F10 cells after 24-h treatment. Cells were treated by calcitriol at 93 (0.24 μM) and 186 ppm (0.48 μM), 60 ppm (0.19 mM) of cisplatin as a positive control and negative control. Relative expression was normalized by GAPDH. Caspase-3, caspase-9, and caspase-8 gene expressions were stimulated, but Bax, Beclin, and RXR alpha did not show significant alteration. Data are presented as average mean and SEM. Significance was considered * P<0.05.