05 May 2022: Laboratory Research
Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16–F10 Cells
Eva Krisna Sutedja 1ABDEF , Daniar Amarassaphira 2ABF , Hanna Goenawan 34BCEF , Yuni Susanti Pratiwi 34BDE , Nova Sylviana 34BDF , Budi Setiabudiawan 5ABDEF , Oki Suwarsa 1BDEF , Raden Tina Dewi Judistiani 6CEF , Unang Supratman 7BEF , Ronny Lesmana 34ABCDEFG*DOI: 10.12659/MSMBR.935139
Med Sci Monit Basic Res 2022; 28:e935139
Figure 4 (A, B) The effect of calcitriol on the protein expression of apoptosis-related proteins in B16–F10 cells after 24-h treatment. Cells were treated by calcitriol at 93 (0.24 μM) and 186 ppm (0.48 μM), 60 ppm (0.19 mM) of cisplatin as a positive control and negative control. Relative protein expression was normalized by β-actin. Caspase-3, caspase-9, caspase-8, Beclin, and poly adenosine diphosphate-ribose polymerases (PARP) protein levels were stimulated. Data are presented as average mean and SEM. Significance was considered * P<0.05.