Figure 1 Identification of FMS-like tyrosine kinase 3 (FLT3)-internal tandem duplication (ITD) mutation by PCR. (A) Locations and orientations of primers used for identification of FLT3-ITD mutation. Primers 11F and 12R were used to amplify FLT3-ITD mutant and FLT3-wild type at exons 14–15. The 329 bp fragment indicates the wild type while the fragment larger than 329 bp indicates the FLT3-ITD mutation. TM – transmembrane domain; JM – juxtamembrane domain; TKD – tyrosine kinase domain. (B) A representative 2% agarose gel electrophoresis of PCR products. Lane 1 and lane 2 are positive and negative control for the FLT3-ITD mutation, respectively. Lanes 3–5 are PCR products of patients with acute myeloid leukemia. Lane 3 shows the 329 bp fragment indicating the wild type. Lane 4 & 5 show the 329 bp and the fragment larger than 329 bp (>329) indicating FLT3-ITD mutation. M indicates 100 bp DNA ladder.