Figure 2 Identification of FMS-like tyrosine kinase 3 (FLT3)-tyrosine kinase domain (TKD) mutation by PCR technique followed by EcoRV digestion. (A) Locations and orientations of primers. Forward primer, F1, and reverse primer, R1, specifically to codon 20 of TKD2 domain were used to produce a 555 bp fragment. Codons 835 and 836 are encoded by nucleotides GATATC which form the EcoRV restriction site. The PCR product of wild type patients were digested by EcoRV into 2 fragments, 260 and 295 bp fragments. The acute myeloid leukemia patients with FLT3-TKD mutations, the PCR product shows 555 bp undigested fragment. TM – transmembrane domain; JM – juxtamembrane domain; TKD – tyrosine kinase domain. (B) A representative 2% agarose gel electrophoresis of PCR products among AML patients after EcoRV digestion. Lane 1 shows uncut PCR Product. Lane 2 & 3, wild type patients show PCR-RFLP fragments which the EcoRV digested produced 260 and 295 bp fragments. Lanes 4 and 5 are positive for FLT3-internal tandem duplication (ITD) mutation which show 555 bp undigested fragments. M indicates the 100 bp DNA ladder.