01 August 2005
Dual role of VEGF family members in the pathogenesis of head and neck cancer(HNSCC): possible link between angiogenesis and immune tolerance.
Laura Strauss, Dagmar Volland, Martin Kunkel, Torsten ReichertMed Sci Monit 2005; 11(8): BR280-292 :: ID: 201014
Abstract
Background: Antigen presenting cells, in particular dendritic cells (DCs),are critical elements in antitumor immunity induction. Some of the angiogenic factors released by tumorand stroma cells, including vascular endothelial growth factor (VEGF), are thought to affect DC function.Material/Methods: The expression of Vascular Endothelial Growth Factor (VEGF) isoforms VEGF-A (121, 145,165,189, 206), VEGF-C and VEGF-D were determined by immunohistochemistry, Western blotting and ELISA in 46patients with Head and Neck Squamous Cell Carcinoma (HNSCC), 30 healthy donors, and two HNSCC tumor celllines (PCI-1 and PCI-13). Results: Increased expression of VEGF-A and VEGF-C was found in tumor tissuescompared to normal epithelium (P=0.001). However, VEGF-D levels were decreased in patients with cervicalnodal metastasis as compared to patients with negative lymph node status. VEGF-A plasma levels were increasedin patients with lymph node metastasis (266 pg/ml) compared to patients with negative lymph node status(19.8 pg/ml). Multivariate analysis demonstrated that VEGF-A correlated with microvessel density (P=0.01),disease progression (P=0.038), a reduced number of local and peripheral mature dendritic cells (DC) (P=0.015)and an increased number of peripheral immature DCs (P=0.05). DCs incubated with tumor supernatant orVEGF-A differentiated into immature DCs and did not develop full allostimulatory activity. AllogenicT cells, when co-cultured with these immature DCs, expressed the T regulatory cell marker CD25, CTLA-4,and CD45Ro, and secreted TGF-beta, VEGF-A and IL-10. Conclusions: Taken together, our results identifyVEGF-A as a multifunctional factor involved in angiogenesis, tumor progression, immunosuppression andimmune tolerance.
Keywords: Head and Neck Neoplasms - blood supply, Head and Neck Neoplasms - immunology, Vascular Endothelial Growth Factors - classification, Cell Count, Coculture Techniques, Dendritic Cells - metabolism, Disease Progression, Head and Neck Neoplasms - pathology, Immune Tolerance, Macrophages - immunology, Mucous Membrane - metabolism, Neovascularization, Pathologic, Phenotype, Protein Isoforms - metabolism, T-Lymphocytes - immunology, Vascular Endothelial Growth Factors - metabolism
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Preoperative Blood Transfusion Requirements for Hemorrhoidal Severe Anemia: A Retrospective Study of 128 Pa...Med Sci Monit In Press; DOI: 10.12659/MSM.943126
12 Mar 2024 : Clinical Research
Tissue Inhibitors of Metalloproteinase 1 (TIMP-1) and 3 (TIMP-3) as New Markers of Acute Kidney Injury Afte...Med Sci Monit In Press; DOI: 10.12659/MSM.943500
12 Mar 2024 : Review article
Optimizing Behçet Uveitis Management: A Review of Personalized Immunosuppressive StrategiesMed Sci Monit In Press; DOI: 10.12659/MSM.943240
12 Mar 2024 : Clinical Research
Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe PsoriasisMed Sci Monit In Press; DOI: 10.12659/MSM.943360
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952