01 March 2016 : Laboratory Research
miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
Jianghui YingACE, Yunfeng LiuBD, Ruijin YangCG, Yong ZhangBE, Jianjun XuAEDOI: 10.12659/MSM.897443
Med Sci Monit 2016; 22:697-701
Abstract
BACKGROUND: Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts.
MATERIAL AND METHODS: Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration.
RESULTS: miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts.
CONCLUSIONS: Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts.
Keywords: Burns - pathology, Up-Regulation - genetics
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