05 May 2022 : Laboratory Research
Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16-F10 Cells
Eva Krishna Sutedja1ABDEF, Daniar Amarassaphira2ABF, Hanna Goenawan34BCEF, Yuni Susanti Pratiwi34BDE, Nova Sylviana34BDF, Budi Setiabudiawan5ABDEF, Oki Suwarsa1BDEF, Raden Tina Dewi Judistiani6CEF, Unang Supratman7BEF, Ronny Lesmana34ABCDEFG*DOI: 10.12659/MSMBR.935139
Med Sci Monit Basic Res 2022; 28:e935139
Supplementary Table 1 Primers sequence.
| Primer | Forward | Reverse | Annealing temperature (°C) |
|---|---|---|---|
| VDR | GCTGATCGAACCCCTCATAA | TTCTGGATCATCTTGGCGTA | 58 |
| Bax | ATGCGTCCACCAAGAAGCTGA | AGCAATCATCCTCTGCAGCTCC | 61 |
| Bcl2 | TTCGCAGCGATGTCCAGTCAGCT | TGAAGAGTTCTTCCACCACCGT | 60 |
| RXRa | GAGGTGGAGTCCACCAGCA | AAGCTCGGCTTCCAGAATCTA | 59 |
| Caspase 3 | TGTCATCTCGCTCTGGTACG | TCCCATAAATGACCCCTTCA | 60 |
| Caspase 8 | GACTGCAACCGAGAGG | GCAGGCTCAAGTCATC | 54 |
| Caspase 9 | AGTTCCCGGGTGCTGTCTAT | GCCATGGTCTTTCTGCTCAC | 60 |
| GAPDH | TGC CAG CCT CGT CTC ATA G | ACT GTG CCG TTG AAC TTG C | 58 |